期刊
JOURNAL OF MICROBIOLOGY
卷 60, 期 8, 页码 823-831出版社
MICROBIOLOGICAL SOCIETY KOREA
DOI: 10.1007/s12275-022-2095-y
关键词
Salmonella Typhimurium; protein acetylation; pta; pat
类别
资金
- Basic Science Research Program through the National Research Foundation of Korea (NRF) - Ministry of Science, ICT and Future Planning [NRF-2018R1D1A1A02048280, NRF-2020R1I1A-3072234, NRF-2021R1C1C2005724, NRF-2020M3A9H5104235, NRF-2021R1I1A1A-01043879]
- Korea University
Deficiency in protein acetylation affects the pathogenicity and replication ability of Salmonella Typhimurium, possibly by regulating the binding affinity of nucleoid-associated protein H-NS to virulence genes.
Salmonella Typhimurium is a Gram-negative facultative pathogen that causes a range of diseases, from mild gastroenteritis to severe systemic infection in a variety of animal hosts. S. Typhimurium regulates virulence gene expression by a silencing mechanism using nucleoid-associated proteins such as Histone-like Nucleoid Structuring protein (H-NS) silencing. We hypothesize that the posttranslational modification, specifically protein acetylation, of proteins in gene silencing systems could affect the pathogenic gene expression of S. Typhimurium. Therefore, we created acetylation-deficient mutant by deleting two genes, pat and pta, which are involved in the protein acetylation pathway. We observed that the pat and pta deletion attenuates mouse virulence and also decreases Salmonella's replication within macrophages. In addition, the Delta pat Delta pta strain showed a decreased expression of the horizontally-acquired virulence genes, mgtC, pagC, and ugtL, which are highly expressed in low Mg2+. The decreased virulence gene expression is possibly due to higher H-NS occupancy to those promoters because the pat and pta deletion increases H-NS occupancy whereas the same mutation decreases occupancy of RNA polymerase. Our results suggest that Pat- and Pta-mediated protein acetylation system promotes the expression of virulence genes by regulating the binding affinity of H-NS in S. Typhimurium.
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