4.1 Article

Bioprinting of heterogeneous and multilayered cell-hydrogel constructs using continuous multi-material printing and aerosol-based crosslinking

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STAR PROTOCOLS
卷 3, 期 2, 页码 -

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ELSEVIER
DOI: 10.1016/j.xpro.2022.101303

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  1. National Research Foundation of Korea (NRF) - Korean Government (MSIT) [NRF-2019R1A2B5B03070494]

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Bioprinting is a powerful biofabrication technique that mimics physiological environments and functions. A continuous multiple-material bioprinting system is described that can replicate structurally complex and biologically functional microphysiological systems such as a tumor microenvironment.
Bioprinting is a powerful biofabrication technique that mimics physiological en-vironments and functions. Here, we describe a protocol to set up a continuous multiple-material bioprinting system that can replicate structurally complex and biologically functional microphysiological systems such as a tumor microen-vironment. Although this bioprinting system uses a limited crosslinking agent, it is a versatile and advanced continuous multi-material printing technique.For complete details on the use and execution of this protocol, please refer to Lee et al. (2021).

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