Generation of precision-cut slice cultures of human placenta

We present a protocol to generate an advanced ex vivo model of human placenta. We use a vibrating tissue slicer to obtain precision-cut slices representative of the entire thickness of human placenta. This approach delivers standardized cultures with a preserved microstructure and cellular composition comparable to the native tissue. We applied this system to study SARS-CoV-2 infection at the maternal-fetal interface. Moreover, this system can be used to investigate the basic functions of the human placenta in health and disease. For complete details on the use and execution of this protocol, please refer to Fahmi et al. (2021).

Automated summary

This study presents an advanced ex vivo model of human placenta using precision-cut slices obtained through a vibrating tissue slicer. The generated standardized cultures maintain the microstructure and cellular composition comparable to native tissue. The system was applied to study SARS-CoV-2 infection at the maternal-fetal interface and can be used to investigate the basic functions of the human placenta in health and disease.