Flow cytometry analysis of endothelial cells and subsets of exhausted CD8+ T cells in murine tumor models

Article Oncology

Tumor-associated high endothelial venules mediate lymphocyte entry into tumors and predict response to PD-1 plus CTLA-4 combination immunotherapy

Assia Asrir et al.

Summary: Tumor-associated high endothelial venules (TA-HEVs) are crucial for recruiting lymphocytes into tumors, and their presence is associated with better response and survival in anti-PD-1/anti-CTLA-4 treatment. TA-HECs co-express specific proteins and selectins, which attract lymphocytes to infiltrate tumors. Understanding the mechanism of lymphocyte trafficking in cancer immunity and immunotherapy is important for improving treatment outcomes.

CANCER CELL (2022)

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Article Immunology

PD-1-specific Blocking antibodies that deplete PD-1+ T cells present an inconvenient variable in preclinical immunotherapy experiments

Fanny Polesso et al.

Summary: Therapeutic antibodies blocking PD-1/PD-L1 interaction have shown success in cancer treatment, but different antibody isotypes and species can impact the outcome. This study demonstrates that anti-PD-1 therapy in mice may lead to unintended target cell depletion and emphasizes the importance of considering antibody clones in experimental design and interpretation. Competing anti-PD-1 clones can be used to detect PD-1-expressing cells despite the presence of blocking antibodies, providing valuable insights for future studies.

EUROPEAN JOURNAL OF IMMUNOLOGY (2021)

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Review Peripheral Vascular Disease

High endothelial venules (HEVs) in immunity, inflammation and cancer

Lucas Blanchard et al.

Summary: High endothelial venules (HEVs) are essential for lymphocyte circulation and immune surveillance in lymph nodes and secondary lymphoid organs. These specialized blood vessels expand during inflammation and play important roles in chronic inflammatory diseases. MECA-79(+) tumor-associated HEVs also play crucial roles in promoting lymphocyte entry into tumors in cancer immunotherapy.

ANGIOGENESIS (2021)

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Article Cell Biology