期刊
BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY
卷 86, 期 7, 页码 855-864出版社
OXFORD UNIV PRESS
DOI: 10.1093/bbb/zbac058
关键词
alpha-xylosidase; family GH31; soil metagenome; crystal structure
类别
资金
- Japan Society for the Promotion of Science (JSPS) KAKENHI [26850067, 18H02132]
- National Institute of Advanced Industrial Science and Technology
- Grants-in-Aid for Scientific Research [26850067, 18H02132] Funding Source: KAKEN
MeXyl31, a member of GH31 family, is an alpha-xylosidase that degrades xylosyl substrates. Its crystal structure reveals its tetrameric state and substrate recognition mechanism.
MeXyl31, a member of glycoside hydrolase family 31 (GH31), is the alpha-xylosidase isolated from a soil metagenomic library. The enzyme degrades alpha-xylosyl substrate such as isoprimeverose, alpha-d-xylopyranosyl-(1 -> 6)-glucopyranose. The crystal structure of MeXyl31 was determined at 1.80 angstrom resolution. MeXyl31 forms the tetrameric state. The complexed structure with a xylose in the -1 subsite (alpha-xylose binding site) shows that the enzyme strictly recognizes alpha-xylose. Structural comparison between MeXyl31 and its homologue, Aspergillus niger alpha-xylosidase in GH31, gave insights into the positive subsite of MeXyl31. First, in the tetrameric enzyme, two monomers (a catalytic monomer and the adjacent monomer), are involved in substrate recognition. Second, the adjacent monomer composes a part of positive subsites in MeXyl31. Docking simulation and site-directed mutagenesis suggested that the Arg100 from the adjacent monomer is partially involved in the recognizing of a glucopyranose of isoprimeverose.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据