Post-translational site-specific protein azidolation with an azido pyridoxal derivative

An azido pyridoxal derivative (PLAA) was developed as a protein azidolation reagent, with selectivity towards N-terminal glycine. Successful PLAA modification was achieved with small peptides, natural proteins and lab-expressed proteins under mild conditions, offering a unique method for post-synthesis site-specific azidolation of a peptide or protein.

Automated summary

An azido pyridoxal derivative (PLAA) has been developed as a protein azidolation reagent with selectivity towards N-terminal glycine. It successfully modifies small peptides, natural proteins, and lab-expressed proteins under mild conditions, providing a unique method for post-synthesis site-specific azidolation of a peptide or protein.