期刊
ANALYTICAL SCIENCES
卷 38, 期 1, 页码 131-136出版社
SPRINGERNATURE
DOI: 10.2116/analsci.21P146
关键词
Nucleic acid detection; Ag nanoclusters; Molecular beacon-like; Fluorescent biosensor
资金
- Science and Technology Development of Jilin Province [20190303116SF, 202002008JC]
- Industrial Technology Research and Development Project of Jilin Province [2020C028-1]
- Talents Project for Innovation and Entrepreneurship of Jilin province [2020030]
- Key Laboratory of Fine Chemicals of Jilin Province
A low-cost silver nanocluster fluorescent sensor with a DNA template was developed in this study, which detects target DNA using a hairpin DNA sequence and shows a strong relationship between fluorescence intensity and target DNA concentration.
We have developed a type of low-cost, label-free silver nanocluster molecular beacon-like fluorescence sensor with a DNA template. To detect target DNA with this probe, we use a hairpin DNA sequence based on a turn-on strategy. The transformation of hairpin DNA would visibly influence the formation of Ag nanoclusters, such that the stronger fluorescence will be measured with the solution containing target nucleic acids than that without targets nucleic acids. There is a good liner relationship between the fluorescence and the target DNA concentrations, ranging from 1 to 750 nmol L-1. Importantly, the detection sensing platform allows down to 1 nmol L-1, which is much lower than other studies.
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