期刊
BIOMATERIALS ADVANCES
卷 136, 期 -, 页码 -出版社
ELSEVIER
DOI: 10.1016/j.bioadv.2022.212774
关键词
Graphene oxide quantum dots; Nanomaterials; 10-HDA; Anti-inflammation; Metabolomics
资金
- Key Research and Development Program of Zhejiang Province [2021C02048]
- Zhejiang Province Natural Science Foundation [LQ22C170003]
This study investigated the effects of graphene oxide quantum dots (GOQDs) on the immune function of macrophages and found that they promoted the anti-inflammatory effect of 10-HDA and affected amino acid metabolism and lipid metabolism in these cells. These findings provide a basis for the further application of GOQDs in biomedical fields.
Graphene oxide quantum dots (GOQDs) have attracted substantial attention in numerous fields due to their unique physicochemical properties. However, their nanotoxicity and potential for use in biomedicine still require further study. In this work, the effects of GOQD and trans- 10-hydroxy-2-decenoic acid (10-HDA) cotreatment on the immune function of macrophages (RAW264.7 cells) were investigated. In particular, LC/MS-based metabolomics was performed to evaluate the effects of GOQDs on the metabolism of LPS-stimulated macrophages. Herein, we fabricated GOQDs with good dispersibility and a uniform size distribution of approximately 7 nm using a polyimide-pyrolyzed carbon film as the working electrode, a high-voltage graphite electrode as the cathode, and H2O2 as the oxidant. The GOQDs entered the macrophages and emitted green fluorescence under UV irradiation. Cotreatment with GOQDs and 10-HDA induced RAW 264.7 cell proliferation. GOQDs promoted the anti-inflammatory effect of 10-HDA on LPS-stimulated RAW264.7 cells and attenuated the secretion of TNF??, IL-6, and IL-18. The metabolites in RAW264.7 cells treated with GOQDs were significantly different from those in RAW264.7 cells treated with LPS. The enrichment analysis showed that treatment with GOQDs interfered with amino acid metabolism, and lipid metabolism. Our results demonstrate the role of GOQDs in macrophages and provide a basis for their further application in biomedical fields.
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