期刊
TECHNOLOGY IN CANCER RESEARCH & TREATMENT
卷 21, 期 -, 页码 -出版社
SAGE PUBLICATIONS INC
DOI: 10.1177/15330338221106557
关键词
nanomaterials; radioiodine; combination therapy; proliferation
类别
资金
- Project of Tianjin Science and Education Commission [20JCQNJC01610]
- Tianjin Medical University General Hospital (the Departments of Nuclear Medicine and Radiology)
Compelling evidence suggests that nanoparticles play a crucial role in cancer therapy. In this study, synthesized NPs using HSA-MnO2 labeled I-131 were used for imaging and therapy for anaplastic thyroid carcinoma. The experimental results provided a feasible solution for combining radiotherapy and dual-model imaging by nanoparticles for cancer diagnosis and treatment.
Purpose Compelling evidence suggests that nanoparticles (NPs) play a crucial role in cancer therapy. NPs templated with human serum albumin (HSA) has good retention in tumors. Manganese dioxide (MnO2) has been used to enhance the effect of radiotherapy. In this study, synthesized NPs using HSA-MnO2 labeled I-131 to perform both imaging and therapy for anaplastic thyroid carcinoma (ATC). Method HSA-MnO2 was synthesized via HSA using a simple biomineralization method, and then labeled with (NaI)-I-131 by the chloramine T method. The cytotoxicity and biosafety of HSA-MnO2 were evaluated by the MTT test. The proliferation-inhibiting effect of HSA-MnO2-I-131 was evaluated in papillary thyroid cancer cell lines (K1, BCPAP, and KTC) and anaplastic thyroid carcinoma cell lines (Cal62, THJ16T, and ARO). For further translational application in medicine, we established a model of transplantable subcutaneously tumors in BALB\c-nu mice to assess the anti-tumor effect of HSA-MnO2-I-131. The imaging effects of NPs were evaluated by MRI and SPECT/CT. Results The MTT test proved that the HSA-MnO2 had low toxicity. HSA-MnO2-I-131 significantly inhibited the proliferation of PTC and ATC cell lines. In addition, the results unveiled that HSA-MnO2-I-131 exhibited dual-modality MR/SPECT imaging for thyroid cancer visualization. In particular, HSA-MnO2-I-131 had an enhanced T1 signal in MR. Using SPECT/CT, we observed that HSA-MnO2-I-131 had good retention in tumor tissue, which was helpful for the diagnosis and treatment of tumor. In vivo assays indicated that the NPs led to a reduction in radioresistance in the tumor hypoxic microenvironment. Conclusion The nanomaterial had a simple synthesis method, good water solubility and biosafety, and good retention in tumor tissue. Hence, it could be used for SPECT/CT and MR dual mode imaging and therapy with radioiodine of tumor cells. The experimental results provided a feasible solution for combining radiotherapy and dual-model imaging by NPs for cancer diagnosis and treatment.
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