期刊
GREEN CHEMISTRY
卷 24, 期 13, 页码 5263-5279出版社
ROYAL SOC CHEMISTRY
DOI: 10.1039/d2gc01160c
关键词
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资金
- Jiangsu Qing Lan Project
- Young Elite Scientists Sponsorship Program by CAST
- University of Tennessee
This study proposes a method to improve the enzymatic digestibility of diluted acid pretreated bamboo residues (DAP-BR) by using low-cost milk protein. The effects of milk protein on the interactions between lignin and enzymes were investigated using fluorescence emission spectroscopy and surface plasmon resonance (SPR). The results show that milk protein can decrease the adsorption of lignin on cellulase and intervene in the interaction between DAP-MWL and cellulase.
The residual lignin in pretreated biomass significantly hinders the bio-conversion of cellulose into monosaccharides. In this work, a low-cost non-enzymatic protein from defatted milk was proposed to block the lignin-enzyme adsorption sites for improving the enzymatic digestibility of diluted acid pretreated bamboo residues (DAP-BR). The enzymatic hydrolysis results showed that the sugar yield significantly improved from 45.8% to 94.6% with a protein dosage of 100 mg(-1) glucan. Fractions of surface lignin (SL) and residual lignin (MWL) from pretreated biomass were obtained and used to investigate the effects of milk protein on the interactions between lignin and enzymes by fluorescence emission spectroscopy and surface plasmon resonance (SPR). The fluorescence emission results showed that the addition of protein dramatically decreased the quenching efficiency of lignin fractions for cellulase. The SPR results revealed that milk protein decreased the non-productive absorption between lignin fractions and the enzyme, which showed better performance for intervening in the interaction between DAP-MWL and cellulase than that for DAP-SL. In addition, strong interactions between cellulase and lignin fractions of DAP-SL (Delta G(bind) = -139.01 kJ mol(-1)) were demonstrated by a dynamic molecular simulation. In conclusion, this work highlights the potential utilization of low-cost milk to achieve an economically viable biorefinery.
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