期刊
ORGANIC & BIOMOLECULAR CHEMISTRY
卷 20, 期 27, 页码 5423-5433出版社
ROYAL SOC CHEMISTRY
DOI: 10.1039/d2ob00830k
关键词
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资金
- Basic Science Research Program through the National Research Foundation (NRF) of Korea - Ministry of Education [2018-R1A6A1A03025124]
- Bio & Medical Technology Development Program of the NRF of Korea - Ministry of Science ICT [2022-R1F1A1069954, 2022-M3A9H1014157, 2021-M3A9I5030523]
- Korea Health Technology R&D Project of the Korea Health Industry Development Institute (KHIDI) - Ministry of Health & Welfare, Republic of Korea [HI21C0239]
In this study, a new fluorescent nanoprobe BMeS-Ali is developed for the visualization of lipid droplets (LDs). BMeS-Ali shows selective staining ability, excellent biocompatibility, and can be applied to the visualization of human fingerprints.
Lipid droplets (LDs) are crucial biological organelles connected with metabolic pathways in biological systems and diseases. To monitor the locations and accumulation of LDs in lipid-related diseases, the development of a visualization tool for LDs has gained importance. In particular, LD visualization using fluorescent probes has gained attention. Herein, a new fluorescent nanoprobe, BMeS-Ali, is developed that can sense LDs based on an amphiphilic single benzene-based fluorophore (SBBF). BMeS-Ali consists of hydrophilic (-NH2) and hydrophobic (-C12H25) moieties and exists as a micelle nanostructure in aqueous media. BMeS-Ali has a weak fluorescence, but its emission was dramatically enhanced upon exposure to the LD components such as oleic acids (OA) by reassembling its nano-formulation. BMeS-Ali showed a selective LD staining ability and great biocompatibility in cells (cancer cells and stem cells). It also showed a practical sensing ability towards biologically derived lipids and can be applied to the visualization of human fingerprints. We found that the nanoprobe BMeS-Ali has significant potential to serve as a practical dye and sensor for lipids, especially for LD imaging in the biomedical research area and broader industrial applications.
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