CATCH: high specific transcriptome-focused fusion gene variants discrimination

We develop a CRISPR-Cas13a triggered catalytic hairpin assembly (CATCH) approach for accurate and impartial identification of variants by integrating the fusion gene-selected recognition of CRISPR-Cas13a with collateral cleavage-assisted catalytic hairpin assembly amplification. This approach achieved an accuracy of 100% in a pilot experiment involving 34 clinical samples.

Automated summary

This paper introduces a CATCH approach using CRISPR-Cas13a, which combines gene recognition with catalytic hairpin assembly amplification to accurately and impartially identify variants. In a pilot experiment involving 34 clinical samples, this approach achieved 100% accuracy.