期刊
OPTICA
卷 3, 期 4, 页码 452-457出版社
OPTICAL SOC AMER
DOI: 10.1364/OPTICA.3.000452
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资金
- Carl-Zeiss-Stiftung
- Leibniz-Gemeinschaft
Raman spectroscopy is a laser spectroscopic method that reveals detailed chemical information about a sample. Pointwise scanning produces chemical maps of heterogeneous samples such as biological tissue without prior staining. However, the intrinsically low Raman scattering cross-sections result in long acquisition times, despite the high excitation intensity in the focus. Here, we present a novel technique termed light sheet Raman micro-spectroscopy, which is an imaging spectrometer attached to a light sheet illumination microscope that can quickly acquire large hyperspectral Raman images. This method is more than 5 times faster than conventional approaches, while reducing the local excitation intensity over 300 fold. We show a stack of hyperspectral Raman images of a zebrafish eye with 1024 x 1024 x 50 spectra (17 min/slice) to demonstrate how the application of spontaneous Raman micro-spectroscopy can be extended to biomedical research and diagnosis based on directly utilizing the molecular information present in the sample. (C) 2016 Optical Society of America
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