Quantitative Analysis of PFAS in Milk, Infant Formula, and Related Ingredients Using Liquid Chromatography-Tandem Mass Spectrometry

Per- and polyfluoroalkyl substances (PFAS) are a large group of anthropogenic chemicals that have been applied in a wide range of industrial, commercial, and domestic products since the 1950s. Because of their toxicity, persistence, and bioaccumulation potential, PFAS have become global environmental pollutants. Besides the environment, the food chain represents another source of exposure, and the risk to consumers related to the presence of PFAS in foods has recently become of increased interest. In this respect, whole milk, infant formula, and ingredients used in infant formula production represent important foodstuffs that require sensitive methods with reporting limits at low parts per billion (ppb) levels or lower for multiple PFAS. This article summarizes optimization experiments and the validation of a complete workflow, including sample preparation and a liquid chromatography-tandem mass spectrometry (LC-MS/MS) method for the determination of 16 priority PFAS analytes listed by the U.S. Food and Drug Administration (FDA).

Automated summary

This article summarizes optimization experiments and validation of a complete workflow for the determination of PFAS in food using a liquid chromatography-tandem mass spectrometry method. PFAS are a group of anthropogenic chemicals that have become global environmental pollutants due to their toxicity and persistence. Recently, there has been increased interest in the risk to consumers related to the presence of PFAS in foods.