4.6 Article

Highly multiplexed immune repertoire sequencing links multiple lymphocyte classes with severity of response to COVID-19

期刊

ECLINICALMEDICINE
卷 48, 期 -, 页码 -

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ELSEVIER
DOI: 10.1016/j.eclinm.2022.101438

关键词

COVID-19; Immune repertoires; Immune receptor; Clinical course

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  1. Berlin Institute of Health (BIH)

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This study analyzed the immune response of healthy individuals and patients with COVID-19, finding specific immune clone types present in severe cases and more shared clone types in healthy individuals. The findings suggest that adaptive immune receptor repertoire measures are associated with disease severity in COVID-19.
Background Disease progression of subjects with coronavirus disease 2019 (COVID-19) varies dramatically. Understanding the various types of immune response to SARS-CoV-2 is critical for better clinical management of coronavirus outbreaks and to potentially improve future therapies. Disease dynamics can be characterized by deciphering the adaptive immune response. Methods In this cross-sectional study we analyzed 117 peripheral blood immune repertoires from healthy controls and subjects with mild to severe COVID-19 disease to elucidate the interplay between B and T cells. We used an immune repertoire Primer Extension Target Enrichment method (immunoPETE) to sequence simultaneously human leukocyte antigen (HLA) restricted T cell receptor beta chain (TRB) and unrestricted T cell receptor delta chain (TRD) and immunoglobulin heavy chain (IgH) immune receptor repertoires. The distribution was analyzed of TRB, TRD and IgH clones between healthy and COVID-19 infected subjects. Using McFadden's Adjusted R2 variables were examined for a predictive model. The aim of this study is to analyze the influence of the adaptive immune repertoire on the severity of the disease (value on the World Health Organization Clinical Progression Scale) in COVID-19. Findings Combining clinical metadata with clonotypes of three immune receptor heavy chains (TRB, TRD, and IgH), we found significant associations between COVID-19 disease severity groups and immune receptor sequences of B and T cell compartments. Logistic regression showed an increase in shared IgH clonal types and decrease of TRD in subjects with severe COVID-19. The probability of finding shared clones of TRD clonal types was highest in healthy subjects (controls). Some specific TRB clones seems to be present in severe COVID-19 (Figure S7b). The most informative models (McFadden's Adjusted R2=0.141) linked disease severity with immune repertoire measures across all three cell types, as well as receptor-specific cell counts, highlighting the importance of multiple lymphocyte classes in disease progression. Interpretation Adaptive immune receptor peripheral blood repertoire measures are associated with COVID-19 disease severity. Copyright (C) 2022 The Authors. Published by Elsevier Ltd.

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