4.1 Article

Shotgun metagenomic sequencing of the first case of monkeypox virus in Brazil, 2022

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INST MEDICINA TROPICAL SAO PAULO
DOI: 10.1590/S1678-9946202264048

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资金

  1. Medical Research Council-Sao Paulo Research Foundation (FAPESP) CADDE partnership award [MR/S0195/1, FAPESP18/143890]
  2. Wellcome Trust
  3. Royal Society [204311/Z/16/Z]
  4. Bill & Melinda Gates Foundation [INV-034540, INV-034652]
  5. Wellcome ARTIC network [206298]
  6. Rede Corona-omica BR MCTI/FINEP [FINEP 01.20.0029.000462/20, CNPq 404096/2020-4]
  7. CNPq/MCTI [402794/2020-6]
  8. Bill and Melinda Gates Foundation [INV-034652, INV-034540] Funding Source: Bill and Melinda Gates Foundation

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This article reports the first confirmed case of Monkeypox virus infection in Brazil and describes the use of shotgun metagenomic sequencing for genome analysis.
Monkeypox virus (MPXV), a zoonotic virus endemic to the African continent, has been reported in 33 non-endemic countries since May 2022. We report an almost complete genome of the first confirmed case of MPXV in Brazil. Shotgun metagenomic sequencing was completed in 18 hours, from DNA extraction to consensus sequence generation.

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