期刊
MYCOBIOLOGY
卷 44, 期 1, 页码 54-57出版社
KOREAN SOC MYCOLOGY
DOI: 10.5941/MYCO.2016.44.1.54
关键词
Davallialactone; In vitro fertilization; Phellinus baumii; Pig; Spermatozoa
资金
- Basic Science Research Program through the National Research Foundation of Korea (NRF) - Ministry of Education [NRF-2013R1A6A3A04063769]
Davallialactone (DAVA) is a hispidin analogue derived from the medicinal fungus Phellinus baumii. We examined the effect of DAVA on in vitro fertilization (IVF) of pigs. Boar spermatozoa were incubated in fertilization medium with varying concentrations of DAVA, then sperm motility and reactive oxygen species (ROS) level were evaluated. Higher sperm motility was found following the addition of 0.5 or 1 mu M DAVA after incubation than addition of other concentrations or controls. ROS level decreased significantly with the addition of DAVA. The rate of normal fertilization was higher in the presence of 1 mu M DAVA (65.1%) than were those of other concentrations or controls (45.4 similar to 59.4%), and the highest total fertilization rate (mono- and polyspermic oocytes) was observed at 1 mu M DAVA (83%). In conclusion, addition of DAVA to fertilization medium improved sperm motility, and reduced ROS level so as to potentially improve sperm- oocyte binding in IVF, suggesting the potential of a compound isolated from mushrooms in assisted reproductive technology for humans and animals.
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