期刊
CARBOHYDRATE POLYMER TECHNOLOGIES AND APPLICATIONS
卷 4, 期 -, 页码 -出版社
ELSEVIER
DOI: 10.1016/j.carpta.2022.100223
关键词
Endoglucanases; Paper Production; Refining; SEC-MALLS; FTIR; SPR
资金
- Austrian Research Promotion Agency (FFG)
- Government of Lower Austria
- EQ-BOKU VIBT GmbH
- BOKU Core Facility Biomolecular & Cellular Analysis
- BOKU Core Facility ALICE
This study investigated the synergistic effects of enzymes in pulp refining and their relationship with refining performance. The results showed that enzymes with a narrow particle size distribution and a high reduction in molecular weight were beneficial for refining cellulose fibers. Additionally, the combination of endoglucanases with beta-glucosidases or xylanases improved the refining degree and tensile strength of the paper.
Refining of cellulose fibers is essential for reaching desired paper properties, yet highly energy demanding. Enzymes like endoglucanases (e.g. EndoC) are increasingly used to reduce energy consumption during pulp refining. However, prediction of the enzyme effect is still a major concern, considering the high variety of commercially available enzyme formulations, containing a range of different enzymes. In this study, synergisms of xylanases and beta-glucosidases in combination with endoglucanases purified from enzyme formulations were studied and related to their refining performance. Size exclusion chromatography with multi-angle laser light scattering (SEC-MALLS) of carboxymethylcellulose revealed that a narrow size distribution and a high reduction in molecular weight are beneficial characteristics for refining. SEC-MALLS of hardwood pulp resulted in pronounced formation of low molecular weight fractions (log MW 4.3) for most efficient refining enzymes. Application of enzyme formulations and combinations of endoglucanase EndoC with beta-glucosidase or xylanase using Fourier-transform infrared spectroscopy (FTIR) revealed synergistic effects that promoted degradation of amorphous parts of cellulose. Laboratory refining trials on hardwood pulp confirmed the increase in degree of refining and tensile index after addition of xylanase and beta-glucosidase. Surface plasmon resonance (SPR) analysis resulted in strong binding of endoglucanases to regenerated cellulose, which correlated to refining performance.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据