期刊
ACS APPLIED BIO MATERIALS
卷 5, 期 8, 页码 3778-3787出版社
AMER CHEMICAL SOC
DOI: 10.1021/acsabm.2c00323
关键词
sulfonated; copolyaniline; laminin; electrical stimulation; PC12 cell
资金
- Ministry of Science and Technology, Taiwan, R.O.C. [NSC 102-2632-M-033-001-MY3, NSC 104-2113-M-033-001-MY3]
- Center of Nanotechnology, and Institute of Biomedical Technology at CYCU
In this study, sulfonated copolyaniline was used as a material for tissue engineering, and neuronal differentiation was promoted through electrical stimulation and laminin treatment. The results showed that cell adhesion and proliferation increased with the degree of sulfonation, and electrical stimulation and La coating further promoted neuronal cell differentiation.
Sulfonated copolyanilines (SPANs), SPAN-40 and SPAN-75, were prepared and applied in this tissue engineering study. SPAN scaffolds (SPANs) and control group polyaniline (PANI) were synthesized by performing oxidative polymerization. To further research the effects of neuron regeneration, PC12 cells were cultured on as-prepared PANI and SPANs with laminin (La) treatment under electrical stimulation. The effects on PC12 cell differentiation were investigated by controlling the amount of sulfonated groups (-SO3H) in the SPAN chain, the electrical stimulation voltage, and the presence or absence of La coating. The adhesion and proliferation of cells increased with the degree of sulfonation; La and electrical stimulation further promoted neuronal cell differentiation as increased neurite length was demonstrated in the micrograph analyses. In summary, the sulfonated copolyaniline coated with La had the best effect on neuronal differentiation under electrical stimulation, suggesting its potential as a substrate for nerve tissue engineering.
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