NAPT, an unbiased approach for sequential analysis of the protein N- and C-terminome

Protein termini are generally associated with protein stability. Taking advantage of charge state differences between terminal peptides and internal peptides, we developed a new method termed LysargiNase-assisted Analysis of the Protein N- and C-Terminome (NAPT) for selective separation of protein termini. Altogether, we identified 2458 protein N-termini and 3056 protein C-termini from the HeLa cell line.

Automated summary

Protein termini play a crucial role in protein stability. Researchers have developed a new method called NAPT to selectively separate protein termini based on charge state differences. They successfully identified thousands of protein N-termini and C-termini from the HeLa cell line.