期刊
ANALYTICAL METHODS
卷 14, 期 33, 页码 3171-3179出版社
ROYAL SOC CHEMISTRY
DOI: 10.1039/d2ay00931e
关键词
-
资金
- National Institutes of Health [1R01NS105888-01]
In this paper, a 3D-printed multi-modal device was developed to detect NO and ATP in red blood cells. The device achieved simultaneous detection of NO and ATP using amperometry and chemiluminescence reactions, respectively. The optimized device demonstrated high sensitivity and low limits of detection.
In this paper, a 3D-printed multi-modal device was designed and fabricated to simultaneously detect nitric oxide (NO) and adenosine triphosphate (ATP) in red blood cell suspensions prepared from whole blood. Once a sample was injected into the device, NO was first detected (via amperometry) using a three-electrode, dual-opposed, electrode configuration with a platinum-black/Nafion coated gold working electrode. After in-line amperometric detection of NO, ATP was detected via a chemiluminescence reaction, with a luciferin/luciferase solution continuously pumped into an integrated mixing T and the resulting light being measured with a PMT underneath the channel. The device was optimized for mixing/reaction conditions, limits of detection (40 nM for NO and 30 nM for ATP), and sensitivity. This device was used to determine the basal (normoxic) levels of NO and ATP in red blood cells, as well as an increase in concentration of both analytes under hypoxic conditions. Finally, the effect of storing red blood cells in a commonly used storage solution was also investigated by monitoring the production of NO and ATP over a three-week storage time.
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