4.7 Article

Increased Expression of Alpha-, Beta-, and Gamma-Synucleins in Brainstem Regions of a Non-Human Primate Model of Parkinson's Disease

期刊

出版社

MDPI
DOI: 10.3390/ijms23158586

关键词

Parkinson's disease; alpha-synuclein; beta-synuclein; gamma-synuclein; MPTP; non-motor symptoms

资金

  1. Fondation de France
  2. Agence Nationale de la Recherche [PID2019-105136RB-100]
  3. Ministry of Science and Innovation (MICINN)
  4. European Regional Development Fund (ERDF), EU

向作者/读者索取更多资源

Parkinson's disease (PD) is characterized by cell loss in the substantia nigra and the presence of alpha-synuclein-containing neuronal Lewy bodies. This study investigated the expression levels of alpha-, beta-, and gamma-synucleins in brainstem regions involved in PD physiopathology. The results showed increased expression of these synucleins in the PD model macaques, which correlated with cell loss and motor score.
Parkinson's disease (PD) is characterized by cell loss in the substantia nigra and the presence of alpha-synuclein (alpha-syn)-containing neuronal Lewy bodies. While alpha-syn has received major interest in the pathogenesis of PD, the function of beta- and gamma-synucleins (beta-syn and gamma-syn, respectively) is not really known. Yet, these proteins are members of the same family and also concentrated in neuronal terminals. The current preclinical study investigated the expression levels of alpha-, beta-, and gamma-synucleins in brainstem regions involved in PD physiopathology. We analyzed synuclein expression in the substantia nigra, raphe nuclei, pedunculopontine nucleus, and locus coeruleus from control and parkinsonian (by MPTP) macaques. MPTP-intoxicated monkeys developed a more or less severe parkinsonian score and were sacrificed after a variable post-MPTP period ranging from 1 to 20 months. The expression of the three synucleins was increased in the substantia nigra after MPTP, and this increase correlates positively, although not very strongly, with cell loss and motor score and not with the time elapsed after intoxication. In the dorsal raphe nucleus, the expression of the three synucleins was also increased, but only alpha- and gamma-Syn are linked to the motor score and associated cell loss. Finally, although no change in synuclein expression was demonstrated in the locus coeruleus after MPTP, we found increased expression levels of gamma-Syn, which are only correlated with cell loss in the pedunculopontine nucleus. Altogether, our data suggest that these proteins may play a key role in brainstem regions and mesencephalic tegmentum. Given the involvement of these brain regions in non-motor symptoms of PD, these data also strengthen the relevance of the MPTP macaque model of PD, which exhibits pathological changes beyond nigral DA cell loss and alpha-synucleinopathy.

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