期刊
ACS MEASUREMENT SCIENCE AU
卷 2, 期 6, 页码 584-594出版社
AMER CHEMICAL SOC
DOI: 10.1021/acsmeasuresciau.2c00037
关键词
SARS-CoV-2; immunoassay; biosensor; electrochemistry; screen-printed carbon electrodes; fluidic device
资金
- National Institutes of Health on Grant [R01EB031510]
This study presents a low-cost electrochemical immunoassay method for the fast and accurate detection of SARS-CoV-2 nucleocapsid protein. The method utilizes a simple flow device and modified electrodes, enabling automated immunoassay with good selectivity. It has also been successfully applied to detect multiple SARS-CoV-2 variants.
The COVID-19 pandemic focused attention on a pressing need for fast, accurate, and low-cost diagnostic tests. This work presents an electrochemical capillary driven immunoassay (eCaDI) developed to detect SARS-CoV-2 nucleocapsid (N) protein. The low-cost flow device is made of polyethylene terephthalate (PET) and adhesive films. Upon addition of a sample, reagents and washes are sequentially delivered to an integrated screen-printed carbon electrode for detection, thus automating a full sandwich immunoassay with a single end-user step. The modified electrodes are sensitive and selective for SARS-CoV-2 N protein and stable for over 7 weeks. The eCaDI was tested with influenza A and Sindbis virus and proved to be selective. The eCaDI was also successfully applied to detect nine different SARS-CoV-2 variants, including Omicron.
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