期刊
BIOENGINEERED
卷 8, 期 4, 页码 393-403出版社
TAYLOR & FRANCIS INC
DOI: 10.1080/21655979.2016.1235102
关键词
alpha-Amylase; diabetes mellitus; IC50 value; mixed inhibition; phytochemical screening; Vicia faba
资金
- School of Biochemical Engineering, Government of India, New Delhi
Diabetes mellitus is a group of metabolic disorder of glucose metabolism. The proper management of blood glucose level is an indicator in the treatment of this complex pathology. The aim of the present study was to evaluate the inhibitory potential of Vicia faba crude seed extracts on the activities of alpha-amylase. Phytochemical screening, FTIR and HPLC analysis confirmed the presence of a phenolic compound with percentage yield of 9.87% in the acetone extract. Acetone extract of seed had highest inhibitory potential against porcine alpha-amylase (IC50 value of 2.94mg/mL). Kinetic analysis revealed that the acetone and methanol extract displayed mixed mode of inhibition toward -amylase. In-silico analysis was agreement with in-vitro studies in which phenolic compounds (catechin, epicatechin, gallic acid, and proanthocyanidin) showed more negative free energy against standard drug (acarbose) and bound with catalytic residues of alpha-amylase. These results might be due to the synergistic action of constituents present in seed extract or acting separately.
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