Monitoring nucleic acid amplification process using a UiO-66-NH2-based fluorescent sensor

Herein, we developed a nucleic acid amplification process monitoring scheme by the use of UiO-66-NH2, in which pyrophosphate ion (PPi) released from the amplification can competitively coordinate with Zr to weaken the ligand-to-metal charge transfer and cause fluorescence recovery, thus reflecting the amplification process. This is the first attempt at using a MOF for monitoring the nucleic acid amplification process.

Automated summary

In this study, we developed a monitoring scheme for nucleic acid amplification process using UiO-66-NH2. The release of pyrophosphate ion (PPi) during amplification competitively coordinates with Zr, weakening the ligand-to-metal charge transfer and causing fluorescence recovery, thus indicating the progress of amplification.