期刊
ANALYTICAL METHODS
卷 14, 期 36, 页码 3598-3604出版社
ROYAL SOC CHEMISTRY
DOI: 10.1039/d2ay00689h
关键词
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资金
- National Key Research and Development Program of China [2019YFC1606101]
- National Natural Science Foundation of China [22174166, 21976213]
A highly sensitive and selective RGB color analysis method for formaldehyde detection was developed using a DNA functionalized gold nanoparticle probe. The concentration of formaldehyde can be estimated by observing the color change of the solution and using a smartphone application. This method has the potential for real-time on-site determination of formaldehyde.
A highly sensitive and selective RGB color analysis for the detection of formaldehyde (FA) was developed by using a DNA functionalized gold nanoparticle (AuNPs-DNA) probe. When complementary oligonucleotides (oligo 2 and oligo 3) and a silver ion (Ag+) were added to the AuNPs-DNA solution, triplex DNA was formed, resulting in the aggregation of AuNPs, and accompanied by a solution color change from red to purple. With the addition of formaldehyde, it reacted with Ag+, decreased the stability of triplex DNA between AuNPs-DNA, induced the dispersion of AuNPs, and the color of AuNPs recovered to red. Therefore, the formaldehyde concentration could be estimated with the RGB (red, green, blue) values of the AuNP solution by using a smartphone application (APP). The R value of the system was proportional to the concentration of formaldehyde within the range of 0.23-4.50 mg L-1, with a detection limit of 0.14 mg L-1. The method has been successfully applied to detect the residues of formaldehyde in vegetable samples and has the potential of the on-site determination of formaldehyde.
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