期刊
ACS APPLIED BIO MATERIALS
卷 5, 期 9, 页码 4294-4300出版社
AMER CHEMICAL SOC
DOI: 10.1021/acsabm.2c00493
关键词
fluorescent probes; nitroreductase; tunable sensitivity; hypoxia; ultrasensitivity
资金
- National Institute of General Medical Sciences of the National Institutes of Health [R15GM114751, 2R15GM114751-02]
Three near-infrared fluorescent probes A, B, and C have been developed to identify hypoxia via nitroreductase determinations. Theoretical calculations suggest the probes are not planar, and photon absorptions result in electron density transitions.
Three fluorescent probes A, B, and C that function in the near-infrared wavelengths and utilize pseudo xanthene platforms with an oxygen atom at the 10-position replaced by a [Me-N](2-) group have been created to identify hypoxia via nitroreductase determinations at the 0.04, 0.10, and 0.19 ng/mL levels. Theoretical calculations suggest that the probes are not planar due to steric interactions. Absorptions of photons result in the transition of electron density from the indoline moieties to delocalized orbitals on the anthranilic section, ending up on the nitro groups of the electron-poor (i.e., nonreduced) probes (i.e., A, B, and C), whereas those for the more electron-rich (i.e., reduced) probes consisted of movement from the indoline groups to the right side of the anthranilic sections, resulting in a shift in absorption.
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