期刊
OPEN MEDICINE
卷 17, 期 1, 页码 1455-1465出版社
DE GRUYTER POLAND SP Z O O
DOI: 10.1515/med-2022-0549
关键词
gallstone; liver failure; hepatitis B virus; single-nucleotide polymorphism
资金
- Fujian Provincial Natural Science Foundation [2010J01118]
- Fuzhou Health Science and Technology Plan Project [2020-S-wq7]
Genetic variations in UGT1A1 gene are associated with lithogenic risk factor for gallstone formation. This study reveals that UGT1A1*27 and UGT1A1*28 variants are significantly increased in patients with gallstone-related hepatic failure and HBV infection compared to healthy individuals.
Genetic variation in UDP-glucuronosyltransferase 1A1 gene (UGT1A1) is a lithogenic risk factor for gallstone formation. This study aimed to assess genotype and allele frequencies of common UGT1A1 variants in patients with gallstone and hepatitis B virus (HBV)-related hepatic failure. This study enrolled 113 healthy individuals (CTRL), 54 patients with HBV infection (HBV), 134 patients with gallstone-free hepatic failure and HBV infection, and 34 patients with gallstone-related hepatic failure and HBV infection (GRHF). Peripheral venous blood samples were collected for genomic DNA isolation. Polymerase chain reaction amplification was carried out for UGT1A1, followed by direct sequencing. Analysis for genotype and allele frequencies of UGT1A1 variants (UGT1A1*6, UGT1A1*27, UGT1A1*28, and UGT1A1*60) was performed. The allele distributions of the four groups did not deviate from Hardy-Weinberg equilibrium. Allele (A) and genotype (CA) frequency distributions of UGT1A1*27 were significantly different between GRHF and CTRL, or between GRHF and HBV. GRHF and CTRL exhibited significant differences in allele (A) and genotype (CA) frequency distributions of UGT1A1*28. Linkage disequilibrium analysis suggested that haplotype G-G-[TA]7-T may be associated with gallstone in HBV-related hepatic failure. Our data reveal that UGT1A1*27 and UGT1A1*28 variants are significantly observed in patients with GRHF compared to healthy individuals.
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