期刊
RSC ADVANCES
卷 12, 期 39, 页码 25342-25353出版社
ROYAL SOC CHEMISTRY
DOI: 10.1039/d2ra04045j
关键词
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资金
- Wrocaw University of Science and Technology
- Universita degli Studi di Sassari (research fund Fondo di Ateneo per la ricerca 2020)
The study aims to develop a biosensor-based system for detecting epinephrine using a poly-thiophene derivative and tyrosinase as the recognition element. Comparison of two electroanalytical techniques showed that differential pulse voltammetry (DPV) exhibited a wider linear range and lower detection limit compared to chronoamperometry (CA). The prepared biosensor system demonstrated good parameters and high selectivity for epinephrine detection, making it suitable for pharmaceutical applications.
The main goal of the presented study was to design a biosensor-based system for epinephrine (EP) detection using a poly-thiophene derivative and tyrosinase as a biorecognition element. We compared two different electroanalytical techniques to select the most prominent technique for analyzing the neurotransmitter. The prepared biosensor system exhibited good parameters; the differential pulse (DPV) technique presented a wide linear range (1-20 mu M and 30-200 mu M), with a low detection limit (0.18 nM and 1.03 nM). In the case of chronoamperometry (CA), a high signal-to-noise ratio and lower reproducibility were observed, causing a less broad linear range (10-200 mu M) and a higher detection limit (125 nM). Therefore, the DPV technique was used for the calculation of sensitivity (0.0011 mu A mM(-1) cm(-2)), stability (49 days), and total surface coverage (4.18 x 10(-12) mol cm(-2)). The biosensor also showed very high selectivity in the presence of common interfering species (i.e. ascorbic acid, uric acid, norepinephrine, dopamine) and was successfully applied for EP determination in a pharmaceutical sample.
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