Fast and high-fidelity in situ 3D imaging protocol for stem cells and niche components for mouse organs and tissues

Article Hematology

Autophagy in mesenchymal progenitors protects mice against bone marrow failure after severe intermittent stress

Theresa Landspersky et al.

Summary: This study identifies Wnt5a as a crucial factor for niche-dependent hematopoiesis by regulating actin stress fiber orientation and autophagy in mesenchymal progenitor and stem cells. The loss of Wnt5a leads to bone marrow failure and increased mortality. Depleted Wnt5a in niche cells results in defective actin stress fiber orientation, impaired autophagy, and elevated oxidative stress. Pharmacological intervention to attenuate CDC42 activation alleviates these defects and rescues hematopoiesis in mice. Autophagy plays a critical role in supporting hematopoiesis in the niche during stress and in human diseases characterized by peripheral cytopenias and hypocellular BM.

BLOOD (2022)

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Article Biochemical Research Methods

Optimized immunofluorescence staining protocol for imaging germinal centers in secondary lymphoid tissues of vaccinated mice

Sigrid Fra-Bido et al.

Summary: An optimized immunofluorescence staining protocol is presented for imaging germinal center structures in fixed/frozen spleen sections from ChAdOx1 nCoV-19 immunized mice, which can be adapted to identify other cell types within secondary lymphoid tissues. For more information on using this protocol to study immune responses to the COVID vaccine ChAdOx1 nCoV-19, refer to Silva-Cayetano et al. (2020).

STAR PROTOCOLS (2021)

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Article Cell Biology