4.1 Review

A review of protocols for human iPSC culture, cardiac differentiation, subtype-specification, maturation, and direct reprogramming

相关参考文献

注意:仅列出部分参考文献,下载原文获取全部文献信息。
Article Cardiac & Cardiovascular Systems

Identification of Drug Transporter Genomic Variants and Inhibitors That Protect Against Doxorubicin-Induced Cardiotoxicity

Tarek Magdy et al.

Summary: This study demonstrates the cardioprotective effect of the SLC28A3 locus using human induced pluripotent stem cell model. It also identifies a novel cardioprotective single nucleotide polymorphism and a competitive inhibitor, desipramine, against doxorubicin-induced cardiotoxicity.

CIRCULATION (2022)

Article Medicine, Research & Experimental

Enhancing cardiac reprogramming via synthetic RNA oligonucleotides

Jiabiao Hu et al.

Summary: The synthetic RNA oligonucleotide ICR2 enhances the efficiency of reprogramming fibroblasts into cardiomyocytes, reduces the risk of inflammatory reactions, and may potentially be used as a therapeutic agent to improve cardiac reprogramming efficiency.

MOLECULAR THERAPY-NUCLEIC ACIDS (2021)

Article Cardiac & Cardiovascular Systems

Heart Disease and Stroke Statistics-2021 Update A Report From the American Heart Association

Salim S. Virani et al.

Summary: The American Heart Association, in partnership with the National Institutes of Health, publishes annual statistics on heart disease, stroke, and cardiovascular risk factors, including the impact of health behaviors and factors on cardiovascular health. The Statistical Update covers a range of clinical heart and circulatory conditions, providing data on quality of care, procedures, and economic costs. It serves as a critical resource for various stakeholders seeking the best available data on these factors and conditions.

CIRCULATION (2021)

Article Cardiac & Cardiovascular Systems

Human Engineered Heart Tissue Patches Remuscularize the Injured Heart in a Dose-Dependent Manner

Eva Querdel et al.

Summary: The transplantation of engineered heart tissue (EHT) patches resulted in partial remuscularization of the injured heart and improved left ventricular function in a dose-dependent manner in guinea pig and pig models. Human-scale patches were successfully transplanted onto pigs in a proof-of-principle study.

CIRCULATION (2021)

Article Cell & Tissue Engineering

High density bioprocessing of human pluripotent stem cells by metabolic control and in silico modeling

Felix Manstein et al.

Summary: By combining STBR technology with in silico process modeling, challenges in hPSC production were overcome, leading to a rapid 70-fold increase in cell expansion and maintenance of cell quality stability.

STEM CELLS TRANSLATIONAL MEDICINE (2021)

Article Cell & Tissue Engineering

Maturation of Pluripotent Stem Cell-Derived Cardiomyocytes Enables Modeling of Human Hypertrophic Cardiomyopathy

Walter E. Knight et al.

Summary: Researchers developed a method to drive cultured hiPSC-CMs towards maturity, resulting in cells with characteristics of adult human cardiomyocytes. These mature hiPSC-CMs could serve as a useful in vitro platform for studying and characterizing cardiovascular diseases.

STEM CELL REPORTS (2021)

Article Cell Biology

The histone reader PHF7 cooperates with the SWI/SNF complex at cardiac super enhancers to promote direct reprogramming

Glynnis A. Garry et al.

Summary: The histone reader PHF7 cooperates with the SWI/SNF complex at cardiac super enhancers to increase accessibility to core cardiac transcription factors, facilitating direct cardiac reprogramming.

NATURE CELL BIOLOGY (2021)

Article Biochemical Research Methods

A versatile polypharmacology platform promotes cytoprotection and viability of human pluripotent and differentiated cells

Yu Chen et al.

Summary: Through high-throughput screening, a small-molecule cocktail called CEPT was discovered to enhance the survival of human pluripotent stem cells (hPSCs) and their differentiated progeny by simultaneously blocking multiple stress mechanisms. This cocktail showed strong improvements in various key applications in stem-cell research, providing a rationale for the safe and efficient utilization of hPSCs.

NATURE METHODS (2021)

Article Cell & Tissue Engineering

RARG variant predictive of doxorubicin-induced cardiotoxicity identifies a cardioprotective therapy

Tarek Magdy et al.

Summary: A recent study identified a genetic variant associated with anthracycline-induced cardiotoxicity and elucidated its mechanism using hiPSC-CMs. The study found that an RARG agonist can attenuate DIC, providing a new approach for clinical prechemotherapy genetic screening and treatment.

CELL STEM CELL (2021)

Article Biotechnology & Applied Microbiology

Direct reprogramming induces vascular regeneration post muscle ischemic injury

Keerat Kaur et al.

Summary: The study demonstrates the success of reprogramming non-cardiomyocytes into cardiomyocyte-like cells using a modified mRNA gene delivery platform. This approach has shown promising therapeutic potential for cardiac regeneration in conditions such as ischemic heart disease, improving cardiac function and scar size after myocardial infarction.

MOLECULAR THERAPY (2021)

Article Multidisciplinary Sciences

Generation of mature compact ventricular cardiomyocytes from human pluripotent stem cells

Shunsuke Funakoshi et al.

Summary: The study established a differentiation strategy for generating structurally, metabolically, and functionally mature compact ventricular cardiomyocytes from human pluripotent stem cells. These cardiomyocytes exhibited metabolic changes indicative of heart failure when challenged with pathological stimuli.

NATURE COMMUNICATIONS (2021)

Article Cell Biology

Chamber-Specific Protein Expression during Direct Cardiac Reprogramming

Zhentao Zhang et al.

Summary: Forced expression of core cardiogenic transcription factors can reprogram fibroblasts to induced cardiomyocyte-like cells, showing potential for heart regeneration. However, it is uncertain whether chamber-specific cardiomyocytes can be generated through this approach. In vivo cardiac reprogramming post-myocardial infarction induces a ventricular-like phenotype, while in vitro generated iCMs fail to determine their chamber identities, suggesting a potential advantage of in vivo cardiac reprogramming for chamber-matched new cardiomyocyte generation.
Article Biochemical Research Methods

A simple protocol to produce mature human-induced pluripotent stem cell-derived cardiomyocytes

Walter E. Knight et al.

Summary: Culturing human-induced pluripotent stem cell-derived cardiomyocytes in maturation medium containing fatty acids, combined with culture on micropatterned surfaces, can produce cells with a more mature phenotype. The protocol is relatively simple and easily adaptable to new laboratories, as detailed in Knight et al. (2021).

STAR PROTOCOLS (2021)

Article Biochemical Research Methods

Process control and in silico modeling strategies for enabling high density culture of human pluripotent stem cells in stirred tank bioreactors

Felix Manstein et al.

Summary: The protocol outlines controlled expansion of hPSCs in stirred tank bioreactors, achieving high cell densities while reducing culture medium consumption. It provides a detailed blueprint for systematic process development of hPSCs and their progenies.

STAR PROTOCOLS (2021)

Article Biochemical Research Methods

Protocol to measure contraction, calcium, and action potential in human-induced pluripotent stem cell- derived cardiomyocytes

Joe Z. Zhang et al.

Summary: This article presents a protocol for measuring three key functional parameters of hiPSC-CMs, including contractile function, calcium handling, and action potential.

STAR PROTOCOLS (2021)

Article Biochemical Research Methods

An updated protocol for the cost-effective and weekend-free culture of human induced pluripotent stem cells

Davi Marco Lyra-Leite et al.

Summary: The provided protocol details a cost-effective method for culturing hiPSCs using a medium called B8. By offering comprehensive preparation methods and troubleshooting guide, researchers are assisted in conducting cell culture and differentiation experiments more effectively.

STAR PROTOCOLS (2021)

Article Biochemical Research Methods

Massive expansion and cryopreservation of functional human induced pluripotent stem cell-derived cardiomyocytes

Renee G. C. Maas et al.

Summary: This study introduces a cost-effective strategy for expanding high-purity hiPSC-CMs at a large scale, maintaining functionality and enabling a variety of downstream applications.

STAR PROTOCOLS (2021)

Article Biochemical Research Methods

Isolation of human ESC-derived cardiac derivatives and embryonic heart cells for population and single-cell RNA-seq analysis

Federica Santoro et al.

Summary: The combination of population and single-cell RNA sequencing analysis in hESC differentiation and developmental tissues is a powerful approach for studying organ-specific cellular and molecular atlas in human embryogenesis. This protocol covers steps including cardiac-directed differentiation, isolation of cardiac derivatives, isolation of single cardiac cells from human embryonic hearts, and construction of cDNA libraries with Smart-seq2 for comprehensive transcriptional analysis of human developmental samples. Refer to Sahara et al. (2019) for detailed information on the use and execution of this protocol.

STAR PROTOCOLS (2021)

Article Cell Biology

Transferrin improved the generation of cardiomyocyte from human pluripotent stem cells for myocardial infarction repair

Fengzhi Zhang et al.

Summary: This study presented a simple and efficient strategy for cardiac differentiation from human pluripotent stem cells (hPSCs) and conducted functional evaluation in a rat model of myocardial infarction. The addition of transferrin facilitated the transition of hPSCs from TeSR-E8 medium to a simple cardiac differentiation medium, leading to successful initiation of mesoderm differentiation without significant cell death. The generated cardiomyocytes (CMs) showed similar transcriptome and maturation levels to those produced in a traditional medium, and significantly improved physiological function and reduced fibrosis in the infarcted rat heart after transplantation.

JOURNAL OF MOLECULAR HISTOLOGY (2021)

Review Cardiac & Cardiovascular Systems

Cardiomyocyte maturation: advances in knowledge and implications for regenerative medicine

Elaheh Karbassi et al.

NATURE REVIEWS CARDIOLOGY (2020)

Review Cardiac & Cardiovascular Systems

Cardiomyocyte Maturation New Phase in Development

Yuxuan Guo et al.

CIRCULATION RESEARCH (2020)

Article Cell & Tissue Engineering

Negligible-Cost and Weekend-Free Chemically Defined Human iPSC Culture

Hui-Hsuan Kuo et al.

STEM CELL REPORTS (2020)

Review Cell Biology

A Brief Review of Current Maturation Methods for Human Induced Pluripotent Stem Cells-Derived Cardiomyocytes

Razan Elfadil Ahmed et al.

FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY (2020)

Article Cardiac & Cardiovascular Systems

Intronic CRISPR Repair in a Preclinical Model of Noonan Syndrome-Associated Cardiomyopathy

Ulrich Hanses et al.

CIRCULATION (2020)

Article Biotechnology & Applied Microbiology

MicroRNA-Mediated Direct Reprogramming of Human Adult Fibroblasts Toward Cardiac Phenotype

C. Paoletti et al.

FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY (2020)

Article Multidisciplinary Sciences

Cells of the adult human heart

Monika Litvinukova et al.

NATURE (2020)

Article Cell Biology

Down-regulation of Beclin 1 promotes direct cardiac reprogramming

Li Wang et al.

SCIENCE TRANSLATIONAL MEDICINE (2020)

Article Biochemical Research Methods

An Optimized Protocol for Human Direct Cardiac Reprogramming

Tiffany A. Garbutt et al.

STAR PROTOCOLS (2020)

Article Biochemical Research Methods

Subtype-Directed Differentiation of Human iPSCs into Atrial and Ventricular Cardiomyocytes

Mandy Kleinsorge et al.

STAR PROTOCOLS (2020)

Article Biochemical Research Methods

Generation of Engineered Human Myocardium in a Multi-well Format

Malte Tiburcy et al.

STAR PROTOCOLS (2020)

Article Biochemistry & Molecular Biology

A Platform for Generation of Chamber-Specific Cardiac Tissues and Disease Modeling

Yimu Zhao et al.

Article Multidisciplinary Sciences

Ensuring expression of four core cardiogenic transcription factors enhances cardiac reprogramming

Zhentao Zhang et al.

SCIENTIFIC REPORTS (2019)

Article Biochemical Research Methods

Engineering of human cardiac muscle electromechanically matured to an adult-like phenotype

Kacey Ronaldson-Bouchard et al.

NATURE PROTOCOLS (2019)

Article Cell & Tissue Engineering

Fatty Acids Enhance the Maturation of Cardiomyocytes Derived from Human Pluripotent Stem Cells

Xiulan Yang et al.

STEM CELL REPORTS (2019)

Article Cell & Tissue Engineering

Generation of clinical-grade functional cardiomyocytes from human embryonic stem cells in chemically defined conditions

Yuanqing Tan et al.

JOURNAL OF TISSUE ENGINEERING AND REGENERATIVE MEDICINE (2018)

Article Multidisciplinary Sciences

Advanced maturation of human cardiac tissue grown from pluripotent stem cells

Kacey Ronaldson-Bouchard et al.

NATURE (2018)

Article Multidisciplinary Sciences

Long-Term Maintenance of Human Pluripotent Stem Cells on cRGDfK-Presenting Synthetic Surfaces

Jack W. Lambshead et al.

SCIENTIFIC REPORTS (2018)

Article Cell & Tissue Engineering

Contractile Work Contributes to Maturation of Energy Metabolism in hiPSC-Derived Cardiomyocytes

Baerbel M. Ulmer et al.

STEM CELL REPORTS (2018)

Article Cardiac & Cardiovascular Systems

Metabolic Maturation of Human Pluripotent Stem Cell-Derived Cardiomyocytes by Inhibition of HIF1 alpha and LDHA

Dongjian Hu et al.

CIRCULATION RESEARCH (2018)

Article Medicine, Research & Experimental

Deep phenotyping of human induced pluripotent stem cell-derived atrial and ventricular cardiomyocytes

Lukas Cyganek et al.

JCI INSIGHT (2018)

Article Cardiac & Cardiovascular Systems

Chemical Enhancement of In Vitro and In Vivo Direct Cardiac Reprogramming

Tamer M. A. Mohamed et al.

CIRCULATION (2017)

Article Biotechnology & Applied Microbiology

Sinoatrial node cardiomyocytes derived from human pluripotent cells function as a biological pacemaker

Stephanie I. Protze et al.

NATURE BIOTECHNOLOGY (2017)

Article Cardiac & Cardiovascular Systems

In situ reprogramming to transdifferentiate fibroblasts into cardiomyocytes using adenoviral vectors: Implications for clinical myocardial regeneration

Megumi Mathison et al.

JOURNAL OF THORACIC AND CARDIOVASCULAR SURGERY (2017)

Review Developmental Biology

Mechanisms of retinoic acid signaling during cardiogenesis

Sonia Stefanovic et al.

MECHANISMS OF DEVELOPMENT (2017)

Article Biochemical Research Methods

Differentiation of cardiomyocytes and generation of human engineered heart tissue

Kaja Breckwoldt et al.

NATURE PROTOCOLS (2017)

Article Multidisciplinary Sciences

Functional screening in human cardiac organoids reveals a metabolic mechanism for cardiomyocyte cell cycle arrest

Richard J. Mills et al.

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA (2017)

Article Cardiac & Cardiovascular Systems

Rapamycin and CHIR99021 Coordinate Robust Cardiomyocyte Differentiation From Human Pluripotent Stem Cells Via Reducing p53-Dependent Apoptosis

Xiao-Xu Qiu et al.

JOURNAL OF THE AMERICAN HEART ASSOCIATION (2017)

Article Cell & Tissue Engineering

Bmi1 Is a Key Epigenetic Barrier to Direct Cardiac Reprogramming

Yang Zhou et al.

CELL STEM CELL (2016)

Article Cardiac & Cardiovascular Systems

Extracellular Matrix-Mediated Maturation of Human Pluripotent Stem Cell-Derived Cardiac Monolayer Structure and Electrophysiological Function

Todd J. Herron et al.

CIRCULATION-ARRHYTHMIA AND ELECTROPHYSIOLOGY (2016)

Article Cell & Tissue Engineering

Human Engineered Heart Tissue: Analysis of Contractile Force

Ingra Mannhardt et al.

STEM CELL REPORTS (2016)

Article Cardiac & Cardiovascular Systems

Matrigel Mattress A Method for the Generation of Single Contracting Human-Induced Pluripotent Stem Cell-Derived Cardiomyocytes

Tromondae K. Feaster et al.

CIRCULATION RESEARCH (2015)

Letter Biochemical Research Methods

Chemically defined, albumin-free human cardiomyocyte generation

Xiaojun Lian et al.

NATURE METHODS (2015)

Article Multidisciplinary Sciences

Akt1/protein kinase B enhances transcriptional reprogramming of fibroblasts to functional cardiomyocytes

Huanyu Zhou et al.

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA (2015)

Article Multidisciplinary Sciences

Contractility of single cardiomyocytes differentiated from pluripotent stem cells depends on physiological shape and substrate stiffness

Alexandre J. S. Ribeiro et al.

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA (2015)

Article Multidisciplinary Sciences

Systematic optimization of human pluripotent stem cells media using Design of Experiments

Paulo A. Marinho et al.

SCIENTIFIC REPORTS (2015)

Article Biochemistry & Molecular Biology

MiR-133 promotes cardiac reprogramming by directly repressing Snai1 and silencing fibroblast signatures

Naoto Muraoka et al.

EMBO JOURNAL (2014)

Article Cardiac & Cardiovascular Systems

Tri-iodo-L-thyronine promotes the maturation of human cardiomyocytes-derived from induced pluripotent stem cells

Xiulan Yang et al.

JOURNAL OF MOLECULAR AND CELLULAR CARDIOLOGY (2014)

Article Biochemical Research Methods

Chemically defined generation of human cardiomyocytes

Paul W. Burridge et al.

NATURE METHODS (2014)

Article Multidisciplinary Sciences

Scalable Passaging of Adherent Human Pluripotent Stem Cells

Ying Nie et al.

PLOS ONE (2014)

Article Cardiac & Cardiovascular Systems

Ultrastructural Maturation of Human-Induced Pluripotent Stem Cell-Derived Cardiomyocytes in a Long-Term Culture

Tsukasa Kamakura et al.

CIRCULATION JOURNAL (2013)

Article Biochemical Research Methods

Biowire: a platform for maturation of human pluripotent stem cell-derived cardiomyocytes

Sara S. Nunes et al.

NATURE METHODS (2013)

Article Biochemical Research Methods

Production of hepatocyte-like cells from human pluripotent stem cells

Nicholas R. F. Hannan et al.

NATURE PROTOCOLS (2013)

Article Multidisciplinary Sciences

Reprogramming of human fibroblasts toward a cardiac fate

Young-Jae Nam et al.

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA (2013)

Article Multidisciplinary Sciences

Induction of human cardiomyocyte-like cells from fibroblasts by defined factors

Rie Wada et al.

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA (2013)

Article Cell & Tissue Engineering

Structural and Functional Maturation of Cardiomyocytes Derived from Human Pluripotent Stem Cells

Scott D. Lundy et al.

STEM CELLS AND DEVELOPMENT (2013)

Article Cell & Tissue Engineering

Direct Reprogramming of Human Fibroblasts toward a Cardiomyocyte-like State

Ji-Dong Fu et al.

STEM CELL REPORTS (2013)

Article Cardiac & Cardiovascular Systems

MicroRNA-Mediated In Vitro and In Vivo Direct Reprogramming of Cardiac Fibroblasts to Cardiomyocytes

Tilanthi M. Jayawardena et al.

CIRCULATION RESEARCH (2012)

Article Multidisciplinary Sciences

Heart repair by reprogramming non-myocytes with cardiac transcription factors

Kunhua Song et al.

NATURE (2012)

Article Multidisciplinary Sciences

Robust cardiomyocyte differentiation from human pluripotent stem cells via temporal modulation of canonical Wnt signaling

Xiaojun Lian et al.

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA (2012)

Article Biochemistry & Molecular Biology

Cardiac Induction of Embryonic Stem Cells by a Small Molecule Inhibitor of Wnt/β-Catenin Signaling

Hanmin Wang et al.

ACS CHEMICAL BIOLOGY (2011)

Article Cardiac & Cardiovascular Systems

Small molecule Wnt inhibitors enhance the efficiency of BMP-4-directed cardiac differentiation of human pluripotent stem cells

Yongming Ren et al.

JOURNAL OF MOLECULAR AND CELLULAR CARDIOLOGY (2011)

Article Biochemical Research Methods

NKX2-5eGFPw hESCs for isolation of human cardiac progenitors and cardiomyocytes

David A. Elliott et al.

NATURE METHODS (2011)

Article Biochemical Research Methods

Chemically defined conditions for human iPSC derivation and culture

Guokai Chen et al.

NATURE METHODS (2011)

Article Biochemistry & Molecular Biology

Direct Reprogramming of Fibroblasts into Functional Cardiomyocytes by Defined Factors

Masaki Ieda et al.

Article Cardiac & Cardiovascular Systems

Energy Metabolic Phenotype of the Cardiomyocyte During Development, Differentiation, and Postnatal Maturation

Gary D. Lopaschuk et al.

JOURNAL OF CARDIOVASCULAR PHARMACOLOGY (2010)

Article Multidisciplinary Sciences

Revealing a core signaling regulatory mechanism for pluripotent stem cell survival and self-renewal by small molecules

Yue Xu et al.

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA (2010)

Article Cardiac & Cardiovascular Systems

Bone morphogenetic protein-4 promotes induction of cardiomyocytes from human embryonic stem cells in serum-based embryoid body development

Shunsuke Takei et al.

AMERICAN JOURNAL OF PHYSIOLOGY-HEART AND CIRCULATORY PHYSIOLOGY (2009)

Article Cell & Tissue Engineering

Wnt3a-Induced Mesoderm Formation and Cardiomyogenesis in Human Embryonic Stem Cells

Thanh H. Tran et al.

STEM CELLS (2009)

Article Multidisciplinary Sciences

Albumin-Associated Lipids Regulate Human Embryonic Stem Cell Self-Renewal

Francesc R. Garcia-Gonzalo et al.

PLOS ONE (2008)

Article Multidisciplinary Sciences

Heparin promotes the growth of human embryonic stem cells in a defined serum-free medium

Miho K. Furue et al.

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA (2008)

Article Biotechnology & Applied Microbiology

Cardiomyocytes derived from human embryonic stem cells in pro-survival factors enhance function of infarcted rat hearts

Michael A. Laflamme et al.

NATURE BIOTECHNOLOGY (2007)

Article Biotechnology & Applied Microbiology

Derivation of human embryonic stem cells in defined conditions

TE Ludwig et al.

NATURE BIOTECHNOLOGY (2006)

Article Cardiac & Cardiovascular Systems

Proliferation of cardiomyocytes derived from human embryonic stem cells is mediated via the IGF/PI 3-kinase/Akt signaling pathway

TC McDevitt et al.

JOURNAL OF MOLECULAR AND CELLULAR CARDIOLOGY (2005)

Article Reproductive Biology

Feeder layer- and serum-free culture of human embryonic stem cells

M Amit et al.

BIOLOGY OF REPRODUCTION (2004)