期刊
AGING-US
卷 14, 期 10, 页码 4376-4389出版社
IMPACT JOURNALS LLC
关键词
circRNA; OSCC; cancer immunity; PDL1; Treg
资金
- Basic and Applied Basic Research Foundation of Guangdong Province [2020A1515111039]
- Basic and Applied Basic Research Foundation of Guangzhou [202102021209]
- Clinical Doctor Foundation of Guangzhou Women and Children Medical Center [1600047]
In this study, it was discovered that circRNA has_circ_0069313 is upregulated in oral squamous cancer cells (OSCC) and is associated with poor prognosis. The mechanism of has_circ_0069313's function involves inhibiting miR-325-3p-induced degradation of Foxp3, resulting in immunity escape. Furthermore, has_circ_0069313 acts as an exosomal circRNA and promotes the function of Treg cells by maintaining Foxp3 levels.
Introduction: CircRNAs are engaged in the tumorigenesis and progression of oral squamous cancer cells (OSCC). However, the function and underlying mechanism of circRNAs on tumor-associated immunity escape are largely unknown. Materials and methods: We analyzed the expression pattern of has_circ_0069313 in our in-house database and its correlation with OSCC prognosis. Immunohistochemistry was applied to detected PDL1 expression. RNA fluorescence in situ hybridization was applied to detect subcellular location of circRNA. A luciferase activity assay was used to detect the interaction of has_circ_0069313 and miR-325-3p and its downstream target, Foxp3. Exosomes were collected to detect the exosomal circRNAs and co-culture assays were performed to detect the function of exosomal circRNAs on Tregs. Results: has_circ_0069313 was upregulated in OSCC tissues and predicts poor prognosis. has_circ_0069313 promotes immunity escape through inhibiting miR-325-3p-induced Foxp3 degradation. has_circ_0069313 is an exosomal circRNA and the transfer of has_circ_0069313 to Treg cells promotes the Treg function through maintaining Foxp3 levels. Conclusion: Our results indicate that has_circ_0069313 induces OSCC immunity escape via the miR-325-3p-Foxp3 axis in both OSCC cells and Treg cells.
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