期刊
ACTA CRYSTALLOGRAPHICA SECTION F-STRUCTURAL BIOLOGY COMMUNICATIONS
卷 72, 期 -, 页码 903-910出版社
INT UNION CRYSTALLOGRAPHY
DOI: 10.1107/S2053230X16018781
关键词
hydrolases; amylovoran; low-molecular-weight protein tyrosine phosphatase; kinetics; fire blight; Enterobacteriaceae; Erwinia amylovora
资金
- European Molecular Biology Laboratory [MX-152]
- Fondazione Libera Universita di Bolzano
- Autonomous Province of Bolzano project: 'A structural genomics approach for the study of the virulence and pathogenesis of Erwinia amylovora'
- University of Bologna through the FARB Program (Finanziamenti dell'Alma Mater Studiorum alla Ricerca di Base)
uAmsI is a low-molecular-weight protein tyrosine phosphatase that regulates the production of amylovoran in the Gram-negative bacterium Erwinia amylovora, a specific pathogen of rosaceous plants such as apple, pear and quince. Amylovoran is an exopolysaccharide that is necessary for successful infection. In order to shed light on AmsI, its structure was solved at 1.57 angstrom resolution at the same pH as its highest measured activity (pH 5.5). In the active site, a water molecule, bridging between the catalytic Arg15 and the reaction-product analogue sulfate, might be representative of the water molecule attacking the phospho-cysteine intermediate in the second step of the reaction mechanism.
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