期刊
ACTA CRYSTALLOGRAPHICA SECTION F-STRUCTURAL BIOLOGY COMMUNICATIONS
卷 72, 期 -, 页码 53-58出版社
INT UNION CRYSTALLOGRAPHY
DOI: 10.1107/S2053230X15024152
关键词
[NiFe]-hydrogenase; X-ray diffraction; crystallization; O-2 tolerance
资金
- JST (CREST)
- JSPS [25291038, 24657077]
- Mitsubishi Foundation
- ENEOS Hydrogen Trust Fund
- Grants-in-Aid for Scientific Research [24657077, 15K05566] Funding Source: KAKEN
The purification procedure of Hyd-2-type [NiFe]-hydrogenase from Citrobacter sp. S-77 was improved by applying treatment with trypsin before chromatography. Purified protein samples both with and without trypsin treatment were successfully crystallized using the sitting-drop vapour-diffusion method with polyethylene glycol as a precipitant. Both crystals belonged to space group P2(1), with unit-cell parameters a = 63.90, b = 118.89, c = 96.70 angstrom, beta = 100.61 degrees for the protein subjected to trypsin treatment and a = 65.38, b = 121.45, c = 98.63 angstrom, beta = 102.29 degrees for the sample not treated with trypsin. The crystal obtained from the trypsin-treated protein diffracted to 1.60 angstrom resolution, which is considerably better than the 2.00 angstrom resolution obtained without trypsin treatment. The [NiFe]-hydrogenase from Citrobacter sp. S-77 retained catalytic activity with some amount of O-2, indicating that it has clear O-2 tolerance.
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