期刊
SCIENTIFIC REPORTS
卷 12, 期 1, 页码 -出版社
NATURE PORTFOLIO
DOI: 10.1038/s41598-022-18846-w
关键词
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资金
- Secretaria de Ciencia y Tecnologia-Universidad Nacional de Cordoba (SECYT) [33620180100091CB]
- Ministerio de Ciencia, Tecnologia e Innovacion, Gobierno de Cordoba [GRFT 77/19]
- Agencia Nacional de Promocion Cientifica y Tecnologica Argentina [FONCYT PICT/18-02324]
This study investigates the early events of the replication cycle of Pixuna virus and Rio Negro virus, two mosquito-borne alphaviruses. The findings show that these viruses are primarily internalized through clathrin-mediated endocytosis and the low pH in endosomes is crucial for viral replication. Understanding the entry route of these viruses is important for understanding their pathogenesis and developing new antiviral strategies.
Pixuna virus (PIXV) and Rio Negro virus (RNV) are mosquito-borne alphaviruses belonging to the Venezuelan Equine Encephalitis (VEE) complex, which includes pathogenic epizootic and enzootic subtypes responsible for life-threatening diseases in equines. Considering that the first steps in viral infection are crucial for the efficient production of new progeny, the aim of this study was to elucidate the early events of the replication cycle of these two viruses. To this end, we used chemical inhibitors and the expression of dominant-negative constructs to study the dependence of clathrin and endosomal pH on PIXV and RNV internalization mechanisms. We demonstrated that both viruses are internalized primarily via clathrin-mediated endocytosis, where the low pH in endosomes is crucial for viral replication. Contributing knowledge regarding the entry route of VEE complex members is important to understand the pathogenesis of these viruses and also to develop new antiviral strategies.
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