4.8 Article

Methanol biotransformation toward high-level production of fatty acid derivatives by engineering the industrial yeast Pichia pastoris

相关参考文献

注意:仅列出部分参考文献,下载原文获取全部文献信息。
Article Biotechnology & Applied Microbiology

Comparative proteomics analysis of Pichia pastoris cultivating in glucose and methanol

Rui Hou et al.

Summary: This study conducted a label-free quantitative proteomic analysis of the methanol metabolism in methylotrophic yeast and identified the highly up-regulated expression of the transaldolase isoenzyme, which plays a crucial role in improving methanol biotransformation efficiency in Pichia pastoris.

SYNTHETIC AND SYSTEMS BIOTECHNOLOGY (2022)

Article Biotechnology & Applied Microbiology

Metabolic engineering ofPichia pastorisfor malic acid production from methanol

Feng Guo et al.

Summary: The study demonstrates the application of rational design and gene regulation in engineeringP. pastoristo produce malic acid from sole methanol, achieving a significant increase in malic acid yield through optimization of accumulation modules and gene knockouts. The results provide important guidance and reference for metabolic engineering ofP. pastoristo produce value-added chemicals.

BIOTECHNOLOGY AND BIOENGINEERING (2021)

Article Biotechnology & Applied Microbiology

Screening neutral sites for metabolic engineering of methylotrophic yeast Ogataea polymorpha

Wei Yu et al.

Summary: In this study, genomic neutral sites for gene integration were screened in O. polymorpha via CRISPR-Cas9 technique. 17 potential neutral sites were identified, with fatty alcohol synthetic pathway genes successfully integrated into two of them, resulting in the production of 4.5 mg/L fatty alcohols. This method of neutral sites screening shows promise for streamline metabolic engineering in O. polymorpha and potentially other eukaryotes as well.

SYNTHETIC AND SYSTEMS BIOTECHNOLOGY (2021)

Review Biotechnology & Applied Microbiology

Synthetic Methylotrophy in Yeasts: Towards a Circular Bioeconomy

Jonathan Thomas Fabarius et al.

Summary: Connecting carbon capture and utilization processes with microbial C-1 fermentation substrates can unlock next-generation processes for converting CO2-derived methanol. Using synthetic biology approaches, synthetic methylotrophs can be empowered to produce a variety of commodity chemicals, supporting the establishment of a future circular economy.

TRENDS IN BIOTECHNOLOGY (2021)

Article Biotechnology & Applied Microbiology

Role of cyanobacterial phosphoketolase in energy regulation and glucose secretion under dark anaerobic and osmotic stress conditions

Derrick Shih-Wei Chuang et al.

Summary: This study revealed the important role of phosphoketolase (Xpk) in primary metabolism of cyanobacteria, particularly in energy regulation and dark conditions. Additionally, Xpk is involved in the pathway producing ATP from glycogen to acetate. Deletion of xpk leads to glucose secretion under dark, anaerobic conditions.

METABOLIC ENGINEERING (2021)

Article Microbiology

Improving the Methanol Tolerance of an Escherichia coli Methylotroph via Adaptive Laboratory Evolution Enhances Synthetic Methanol Utilization

R. Kyle Bennett et al.

Summary: Efforts to enhance the methanol tolerance of synthetic methylotrophs are crucial due to the cytotoxic nature of methanol and formaldehyde. This study successfully evolved E. coli strains with significantly improved methanol tolerance and methanol utilization phenotypes. Whole genome sequencing revealed mutations in ribosomal proteins contributing to enhanced methanol tolerance, offering insights into a novel mechanism for alleviating methanol toxicity.

FRONTIERS IN MICROBIOLOGY (2021)

Article Multidisciplinary Sciences

Recombination machinery engineering for precise genome editing in methylotrophic yeast Ogataea polymorpha

Jiaoqi Gao et al.

Summary: The study focuses on improving homologous recombination (HR) activity in O. polymorpha through recombination machinery engineering (rME) and establishing an efficient CRISPR-Cas9 system. By overexpressing HR-related proteins and down-regulating non-homologous end joining (NHEJ), HR rates were significantly increased. This enhanced HR system has allowed for successful homologous integration of large fragments and in vivo assembly of multiple fragments in order to produce fatty alcohols.

ISCIENCE (2021)

Review Biochemistry & Molecular Biology

Established tools and emerging trends for the production of recombinant proteins and metabolites in Pichia pastoris

Sonakshi De et al.

Summary: Pichia pastoris has become the most popular yeast cell factory for producing heterologous proteins, with strong promoters, stable genetic constructs, and a growing collection of strains, tools, and protocols. In addition to small-scale production for biochemical studies and protein characterization, the yeast is also being increasingly used for in vivo biotransformation and metabolic pathway engineering for chemical production.

MICROBIAL CELL FACTORIES-BOOK (2021)

Article Biochemistry & Molecular Biology

Recombination machinery engineering facilitates metabolic engineering of the industrial yeast Pichia pastoris

Peng Cai et al.

Summary: A CRISPR-Cas9 based genome editing platform was established, significantly enhancing the recombination efficiency of P. pastoris and allowing for efficient seamless gene disruption and multiple gene assembly. Additionally, this tool resulted in a 30-fold increase in fatty alcohol production and enabled regulation of gene expression and metabolic pathways under different growth conditions.

NUCLEIC ACIDS RESEARCH (2021)

Article Endocrinology & Metabolism

An orthogonal metabolic framework for one-carbon utilization

Alexander Chou et al.

Summary: The study introduces an alternative approach using synthetic pathways for C1 bioconversion, generating multicarbon products directly from formate, formaldehyde and methanol while being orthogonal to the host metabolic network. Variants of the FORCE pathways were evaluated through thermodynamic and stoichiometric analyses, showing promising results both in vitro and in vivo. This synthetic metabolic pathway demonstrates the potential for C1 compound utilization and integration with host metabolism for synthetic methylotrophy.

NATURE METABOLISM (2021)

Article Chemistry, Multidisciplinary

Biosynthesis of non-animal chondroitin sulfate from methanol using genetically engineered Pichia pastoris

Xuerong Jin et al.

Summary: The study successfully developed a new method for the de novo biosynthesis of non-animal CS using engineered Pichia pastoris cells, producing 182.0 mg L-1 CSA with a sulfation degree of 4.0%. The results suggest that this approach could be used as a green route to produce other sulfated glycosaminoglycans.

GREEN CHEMISTRY (2021)

Review Biotechnology & Applied Microbiology

Synthetic Methylotrophy: A Practical Solution for Methanol-Based Biomanufacturing

Yu Wang et al.

TRENDS IN BIOTECHNOLOGY (2020)

Article Biochemical Research Methods

A Synthetic Malonyl-CoA Metabolic Oscillator in Komagataella phaffii

Jiao Wen et al.

ACS SYNTHETIC BIOLOGY (2020)

Article Biochemistry & Molecular Biology

Converting Escherichia coli to a Synthetic Methylotroph Growing Solely on Methanol

Frederic Y-H Chen et al.

Article Multidisciplinary Sciences

Engineering carboxylic acid reductase for selective synthesis of medium-chain fatty alcohols in yeast

Yating Hu et al.

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA (2020)

Article Multidisciplinary Sciences

Adaptive laboratory evolution of native methanol assimilation in Saccharomyces cerevisiae

Monica I. Espinosa et al.

NATURE COMMUNICATIONS (2020)

Article Biotechnology & Applied Microbiology

Direct production of fatty alcohols from glucose using engineered strains of Yarrowia lipolytica

Lauren T. Cordova et al.

METABOLIC ENGINEERING COMMUNICATIONS (2020)

Article Multidisciplinary Sciences

Constructing a synthetic pathway for acetylcoenzyme A from one-carbon through enzyme design

Xiaoyun Lu et al.

NATURE COMMUNICATIONS (2019)

Review Chemistry, Medicinal

Structure and Function of Δ9-Fatty Acid Desaturase

Kohjiro Nagao et al.

CHEMICAL & PHARMACEUTICAL BULLETIN (2019)

Article Biochemistry & Molecular Biology

Engineering of an oleaginous bacterium for the production of fatty acids and fuels

Hye Mi Kim et al.

NATURE CHEMICAL BIOLOGY (2019)

Article Biotechnology & Applied Microbiology

CRISPR-Cas9-mediated genomic multiloci integration in Pichia pastoris

Qi Liu et al.

MICROBIAL CELL FACTORIES (2019)

Review Biochemical Research Methods

Current advances in engineering tools for Pichia pastoris

Jasmin E. Fischer et al.

CURRENT OPINION IN BIOTECHNOLOGY (2019)

Article Multidisciplinary Sciences

Rewiring carbon metabolism in yeast for high level production of aromatic chemicals

Quanli Liu et al.

NATURE COMMUNICATIONS (2019)

Article Biochemistry & Molecular Biology

Expanding the CRISPR/Cas9 toolkit for Pichia pastoris with efficient donor integration and alternative resistance markers

Astrid Weninger et al.

JOURNAL OF CELLULAR BIOCHEMISTRY (2018)

Review Biotechnology & Applied Microbiology

Engineering strategies for enhanced production of protein and bio-products in Pichia pastoris: A review

Zhiliang Yang et al.

BIOTECHNOLOGY ADVANCES (2018)

Review Energy & Fuels

Experimental parameters affecting the photocatalytic reduction performance of CO2 to methanol: A review

Abul Lais et al.

INTERNATIONAL JOURNAL OF ENERGY RESEARCH (2018)

Article Biotechnology & Applied Microbiology

Heterologous transporter expression for improved fatty alcohol secretion in yeast

Yating Hu et al.

METABOLIC ENGINEERING (2018)

Review Biotechnology & Applied Microbiology

Metabolic engineering of Pichia pastoris

David A. Pena et al.

METABOLIC ENGINEERING (2018)

Article Biochemical Research Methods

High level expression and purification of recombinant human serum albumin in Pichia pastoris

Wen Zhu et al.

PROTEIN EXPRESSION AND PURIFICATION (2018)

Article Biochemistry & Molecular Biology

Reprogramming Yeast Metabolism from Alcoholic Fermentation to Lipogenesis

Tao Yu et al.

Article Biotechnology & Applied Microbiology

Engineering Corynebacterium glutamicum for methanol-dependent growth and glutamate production

Philibert Tuyishime et al.

METABOLIC ENGINEERING (2018)

Article Biotechnology & Applied Microbiology

Synthetic methanol auxotrophy of Escherichia coli for methanol-dependent growth and production

Chang-Ting Chen et al.

METABOLIC ENGINEERING (2018)

Review Energy & Fuels

Barriers and opportunities in bio-based production of hydrocarbons

Yongjin J. Zhou et al.

NATURE ENERGY (2018)

Article Biotechnology & Applied Microbiology

Lipid production in Yarrowia lipolytica is maximized by engineering cytosolic redox metabolism

Kangjian Qiao et al.

NATURE BIOTECHNOLOGY (2017)

Article Mathematical & Computational Biology

GoldenPiCS: a Golden Gate-derived modular cloning system for applied synthetic biology in the yeast Pichia pastoris

Roland Prielhofer et al.

BMC SYSTEMS BIOLOGY (2017)

Review Biotechnology & Applied Microbiology

Towards systems metabolic engineering in Pichia pastoris

Jan-Philipp Schwarzhans et al.

BIOTECHNOLOGY ADVANCES (2017)

Article Biotechnology & Applied Microbiology

Exploring fatty alcohol-producing capability of Yarrowia lipolytica

Guokun Wang et al.

BIOTECHNOLOGY FOR BIOFUELS (2016)

Review Biochemistry & Molecular Biology

Engineering Cellular Metabolism

Jens Nielsen et al.

Article Chemistry, Multidisciplinary

Harnessing Yeast Peroxisomes for Biosynthesis of Fatty-Acid-Derived Biofuels and Chemicals with Relieved Side-Pathway Competition

Yongjin J. Zhou et al.

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY (2016)

Article Biotechnology & Applied Microbiology

Combining metabolic engineering and process optimization to improve production and secretion of fatty acids

Rodrigo Ledesma-Amaro et al.

METABOLIC ENGINEERING (2016)

Review Biotechnology & Applied Microbiology

Microbial production of fatty alcohols

Sandy Fillet et al.

WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY (2016)

Article Multidisciplinary Sciences

Production of fatty acid-derived oleochemicals and biofuels by synthetic yeast cell factories

Yongjin J. Zhou et al.

NATURE COMMUNICATIONS (2016)

Article Biology

Systems-level organization of yeast methylotrophic lifestyle

Hannes Russmayer et al.

BMC BIOLOGY (2015)

Review Biochemical Research Methods

Synthetic methylotrophy: engineering the production of biofuels and chemicals based on the biology of aerobic methanol utilization

William B. Whitaker et al.

CURRENT OPINION IN BIOTECHNOLOGY (2015)

Article Biotechnology & Applied Microbiology

Metabolic engineering of Pichia pastoris for the production of dammarenediol-II

Xin-Bin Liu et al.

JOURNAL OF BIOTECHNOLOGY (2015)

Article Biotechnology & Applied Microbiology

Engineering Escherichia coli for methanol conversion

Jonas E. N. Mueller et al.

METABOLIC ENGINEERING (2015)

Article Biotechnology & Applied Microbiology

Enhanced Production of Fatty Alcohols by Engineering the TAGs Synthesis Pathway in Saccharomyces cerevisiae

Xiaoling Tang et al.

BIOTECHNOLOGY AND BIOENGINEERING (2015)

Article Biotechnology & Applied Microbiology

Production of the sesquiterpenoid (+)-nootkatone by metabolic engineering of Pichia pastoris

Tamara Wriessnegger et al.

METABOLIC ENGINEERING (2014)

Article Biochemistry & Molecular Biology

Formaldehyde induces hyperphosphorylation and polymerization of Tau protein both in vitro and in vivo

Jing Lu et al.

BIOCHIMICA ET BIOPHYSICA ACTA-GENERAL SUBJECTS (2013)

Review Biochemical Research Methods

New opportunities by synthetic biology for biopharmaceutical production in Pichia pastoris

Thomas Vogl et al.

CURRENT OPINION IN BIOTECHNOLOGY (2013)

Article Biotechnology & Applied Microbiology

Engineered fungal polyketide biosynthesis in Pichia pastoris: a potential excellent host for polyketide production

Limei Gao et al.

MICROBIAL CELL FACTORIES (2013)

Article Biotechnology & Applied Microbiology

Determining the Extremes of the Cellular NAD(H) Level by Using an Escherichia coli NAD+-Auxotrophic Mutant

Yongjin Zhou et al.

APPLIED AND ENVIRONMENTAL MICROBIOLOGY (2011)

Article Multidisciplinary Sciences

Increasing NADH oxidation reduces overflow metabolism in Saccharomyces cerevisiae

G. N. Vemuri et al.

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA (2007)

Review Biotechnology & Applied Microbiology

Regulation of methanol utilisation pathway genes in yeasts

Franz S. Hartner et al.

MICROBIAL CELL FACTORIES (2006)