期刊
MICROBIOLOGY RESEARCH
卷 13, 期 3, 页码 556-573出版社
MDPI
DOI: 10.3390/microbiolres13030039
关键词
bacteria; native; soil; cacao; Cd; TEM; FT-IR
类别
资金
- Compania Nacional de Chocolates
In this study, twelve cadmium-tolerant bacteria were isolated from soils of cocoa farms in the Colombian Andes and their ability to tolerate and immobilize cadmium was tested. The results showed that these bacteria could deform their cell morphologies and interact with cadmium through biosorption and bioaccumulation. In addition, the presence of certain bacterial strains could prevent the increase of cadmium concentration in roots, indicating a potential for cadmium immobilization in soil.
Twelve cadmium native bacteria previously isolated in soils of cocoa farms located in the western Colombian Andes (Santander), and tolerant to 2500 mu M CdCl2 (120 mg Cd/L), were chosen in order to test their tolerance and Cd immobilization using liquid culture medium (Nutritive broth) at different concentrations of heavy metals. Furthermore, in the greenhouse experiments, the strains Exiguobacterium sp. (11-4A), Klebsiella variicola sp. (18-4B), and Enterobacter sp. (29-4B) were applied in combined treatments using CCN51 cacao genotype seeds grown in soil with different concentrations of Cd. All bacterial strains' cell morphologies were deformed in TEM pictures, which also identified six strain interactions with biosorption and four strain capacities for bioaccumulation; FT-IR suggested that the amide, carbonyl, hydroxyl, ethyl, and phosphate groups on the bacteria biomass were the main Cd binding sites. In the pot experiments, the concentration of Cd was distributed throughout the cacao plant, but certain degrees of immobilization of Cd can occur in soil to prevent an increase in this level in roots with the presence of Klebsiella sp.
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