4.7 Article

Accurate Cultivar Authentication of Jujube Fruits Using Nano-Fluidic Genotyping of Single Nucleotide Polymorphism (SNP) Markers

期刊

HORTICULTURAE
卷 8, 期 9, 页码 -

出版社

MDPI
DOI: 10.3390/horticulturae8090792

关键词

authentication; Chinese date; DNA fingerprinting; molecular markers; food adulteration

资金

  1. Key Research and Development Projects in Ningxia International cooperation project [2018BFH03015]
  2. Ningxia Nature Foundation project [2021AAC03110]

向作者/读者索取更多资源

Chinese jujube is an economically important fruit tree with high adaptability to various climate conditions. Traditional jujube cultivars in Ningxia, China are highly valued for their unique flavor and quality. This study developed a multi-locus SNP barcoding method for cultivar authentication of jujube fruits, which showed high reliability and practicality. The method has the potential to be applied for the authentication of other stone fruits as well.
Chinese jujube (Ziziphus jujuba Mill.) is an economically important fruit tree that is highly adapted to marginal crop lands and can be grown under a very broad range of climate conditions. Jujube fruits produced from several traditional cultivars in Ningxia, China have enjoyed a premium market price due to their unique flavor and quality attributes. One problem with the high-end jujube market is the adulteration of premium cultivars. The accurate identification of the genetic identity of single jujube fruits is essential for cultivar traceability and authentication. The multi-locus SNP barcoding approach offers an effective solution for cultivar authentication of jujube fruits. To identify variant SNP sequences a nanofluidic array approach was used to test the efficacy of this method with DNA extracted from the fruit pulp of eight jujube cultivars from Ningxia, China. The SNP marker profiles identified the genetic identity of each fruit unambiguously based on multilocus matching and ordination analysis. Results for repeated sampling of multiple fruits from the same tree (using independent DNA extractions) showed a high level of concordance, which demonstrated the reliability of SNP based genotyping platform. This method can handle 100 s to 1000 s of samples per day (based on the platform used). It is robust and cost-effective and has a high potential for its practical application in the jujube industry. The developed method and protocol can be readily applied for botanical authentication of other stone fruits in general.

作者

我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。

评论

主要评分

4.7
评分不足

次要评分

新颖性
-
重要性
-
科学严谨性
-
评价这篇论文

推荐

暂无数据
暂无数据