期刊
3 BIOTECH
卷 6, 期 -, 页码 -出版社
SPRINGER HEIDELBERG
DOI: 10.1007/s13205-015-0338-x
关键词
Naringinase; Aspergillus niger; Immobilization; ART-FTIR (total reflectance Fourier transform infrared); TGA (thermal gravimetric analysis) and biopolymer (grafted alginate gel beads)
资金
- Chemistry of Natural and Microbial Products Department, Biochemistry Department
- Group of Encapsulation and Nanobiotechnology, Centre of Scientific Excellence, National Research Centre (NRC) Giza, Egypt
Naringinase induced from the fermented broth of marine-derived fungus Aspergillus niger was immobilized into grafted gel beads, to obtain biocatalytically active beads. The support for enzyme immobilization was characterized by ART-FTIR and TGA techniques. TGA revealed a significant improvement in the grafted gel's thermal stability from 200 to 300 degrees C. Optimization of the enzyme loading capacity increased gradually by 28-fold from 32 U/g gel to 899 U/g gel beads, retaining 99 % of the enzyme immobilization efficiency and 88 % of the immobilization yield. The immobilization process highly improved the enzyme's thermal stability from 50 to 70 degrees C, which is favored in food industries, and reusability test retained 100 % of the immobilized enzyme activity after 20 cycles. These results are very useful on the marketing and industrial levels.
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