期刊
BIOMATERIALS SCIENCE
卷 10, 期 21, 页码 6315-6325出版社
ROYAL SOC CHEMISTRY
DOI: 10.1039/d2bm01271e
关键词
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资金
- FRM [ECO201806006861]
- GDR CNRS AIM mobility grant
- Oncostarter project of the canceropole CLARA (Agnostic project)
- France Life Imaging (Thera-BODIPY)
- GEFLUC Grenoble Dauphine Savoie
- French National Research Agency [ANR-18-CE18-0012, ANR-11-LABX-0021-01]
- Agence Nationale de la Recherche (ANR) [ANR-18-CE18-0012] Funding Source: Agence Nationale de la Recherche (ANR)
This study investigates the behavior of SWIR-WAZABY-01 fluorophore and its interaction with lipoproteins in vitro and in vivo. The results show that SWIR-WAZABY-01 enhances the fluorescence emission of lipoproteins and promotes tumor accumulation through lipoprotein receptors and passive uptake.
Following intravenous administration, the interaction of fluorescent exogenous molecules with circulating endogenous transporters can influence their photophysical properties as well as their fate and distribution, and possibly their recognition by different cell types. This type of interaction can be used to optimize the drug delivery but also the imaging properties of a compound of interest. In this study, we investigated the behavior of SWIR-WAZABY-01 fluorophore, a water-soluble aza-BODIPY dye emitting in the NIR-II region, both in vitro and in vivo. While the fluorescence emission of SWIR-WAZABY-01 was weak in aqueous solutions, it was intensely magnified in plasma (similar to x30). Further analyses using lipoprotein gel electrophoresis and ultracentrifugation revealed interactions between SWIR-WAZABY-01 and plasma lipoproteins in vitro and ex vivo, in particular with LDL. The tumor uptake mechanism of SWIR-WAZABY-01 was investigated based on the presence of low-density lipoprotein (LDL) receptors and passive tumor uptake. Overall, we found that SWIR-WAZABY-01 interacts with lipoproteins enhancing their NIR-II fluorescence emission, and driving the tumor accumulation with regards to the expression of lipoprotein receptors (LDLR, SR-BI). Moreover, SWIR-WAZABY-01, by exploiting endogenous lipoproteins, arises as a new, potent and relevant tool to efficiently label LDL involved in pathologies.
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