期刊
STEM CELL REPORTS
卷 6, 期 4, 页码 525-538出版社
CELL PRESS
DOI: 10.1016/j.stemcr.2016.03.001
关键词
-
资金
- Funding Programs for DZNE Helmholtz
- TU Dresden CRTD
- DFG [KA2794/3-1, SPP1738]
- MedDrive TU Dresden UKD-Medical Faculty
- Novartis Pharma GmbH
- Volkswagen Foundation Freigeist fellowship
- European Union's sixth Framework Program ESTOOLS
The plasticity of pluripotent stem cells provides new possibilities for studying development, degeneration, and regeneration. Protocols for the differentiation of retinal organoids from embryonic stem cells have been developed, which either recapitulate complete eyecup morphogenesis or maximize photoreceptor genesis. Here, we have developed a protocol for the efficient generation of large, 3D-stratified retinal organoids that does not require evagination of optic-vesicle-like structures, which so far limited the organoid yield. Analysis of gene expression in individual organoids, cell birthdating, and interorganoid variation indicate efficient, reproducible, and temporally regulated retinogenesis. Comparative analysis of a transgenic reporter for PAX6, a master regulator of retinogenesis, shows expression in similar cell types in mouse in vivo, and in mouse and human retinal organoids. Early or late Notch signaling inhibition forces cell differentiation, generating organoids enriched with cone or rod photoreceptors, respectively, demonstrating the power of our improved organoid system for future research in stem cell biology and regenerative medicine.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据