期刊
STEM CELL REPORTS
卷 7, 期 1, 页码 110-125出版社
CELL PRESS
DOI: 10.1016/j.stemcr.2016.05.006
关键词
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资金
- NHLBI Progenitor Cell Biology Consortium, Administrative Coordinating Center [U01HL099997]
- NHLBI Cell Characterization Core
- NHLBI Bioinformatics Core
- National Heart, Lung, and Blood Institute (NHLBI) [U01HL099775]
- National Institute for Child Health and Human Development (NICHD) [R01HD082098]
- National Institute General Medical Sciences (NIGMS) [R01GM110628]
- National Eye Institute (NEI) [R01EY023962]
- [PCBC2012Pilot_01]
The rigorous characterization of distinct induced pluripotent stem cells (iPSC) derived from multiple reprogramming technologies, somatic sources, and donors is required to understand potential sources of variability and downstream potential. To achieve this goal, the Progenitor Cell Biology Consortium performed comprehensive experimental and genomic analyses of 58 iPSC from ten laboratories generated using a variety of reprogramming genes, vectors, and cells. Associated global molecular characterization studies identified functionally informative correlations in gene expression, DNA methylation, and/or copy-number variation among key developmental and oncogenic regulators as a result of donor, sex, line stability, reprogramming technology, and cell of origin. Furthermore, X-chromosome inactivation in PSC produced highly correlated differences in teratoma-lineage staining and regulator expression upon differentiation. All experimental results, and raw, processed, and metadata from these analyses, including powerful tools, are interactively accessible from a new online portal at https://www.synapse.org to serve as a reusable resource for the stem cell community.
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