期刊
STEM CELL REPORTS
卷 6, 期 3, 页码 321-329出版社
CELL PRESS
DOI: 10.1016/j.stemcr.2016.01.014
关键词
-
资金
- NHMRC project [APP1061883]
- Silvia and Charles Senior Medical Viertel Fellowship
The isolation of pure populations of mouse intestinal stem cells (ISCs) is essential to facilitate functional studies of tissue homeostasis, tissue regeneration, and intestinal diseases. However, the purification of ISCs has relied predominantly on the use of transgenic reporter alleles in mice. Here, we introduce a combinational cell surface marker-mediated strategy that allows the isolation of an ISC population transcriptionally and functionally equivalent to the gold standard Lgr5-GFP ISCs. Used on reporter-free mice, this strategy allows the isolation of functional, transcriptionally distinct ISCs uncompromised by Lgr5 haploinsufficiency.
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