期刊
STEM CELL REPORTS
卷 6, 期 3, 页码 422-435出版社
CELL PRESS
DOI: 10.1016/j.stemcr.2016.01.010
关键词
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资金
- Project for Realization of Regenerative Medicine and Support for Core Institutes for iPS Cell Research from Ministry of Education, Culture, Sports, Science and Technology of Japan (MEXT)
- Research Center Network for Realization Research Centers/Projects of Regenerative Medicine (the Program for Intractable Disease Research utilizing disease-specific iPS Cells) from Japan Science and Technology Agency (JST)
- Japan Agency for Medical Research and Development (AMED)
- New Energy and Industrial Technology Development Organization (NEDO)
- Japan Society for Promotion of Science (JSPS)
- MEXT
- Grants-in-Aid for Scientific Research [26830018, 16K08280, 15H04842, 15K15041, 26870074] Funding Source: KAKEN
Modeling of neurological diseases using induced pluripotent stem cells (iPSCs) derived from the somatic cells of patients has provided a means of elucidating pathogenic mechanisms and performing drug screening. T cells are an ideal source of patient-specific iPSCs because they can be easily obtained from samples. Recent studies indicated that iPSCs retain an epigenetic memory relating to their cell of origin that restricts their differentiation potential. The classical method of differentiation via embryoid body formation was not suitable for T cell-derived iPSCs (TiPSCs). We developed a neurosphere-based robust differentiation protocol, which enabled TiPSCs to differentiate into functional neurons, despite differences in global gene expression between TiPSCs and adult human dermal fibroblast-derived iPSCs. Furthermore, neurons derived from TiPSCs generated from a juvenile patient with Parkinson's disease exhibited several Parkinson's disease phenotypes. Therefore, we conclude that TiPSCs are a useful tool for modeling neurological diseases.
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