期刊
FOOD & FUNCTION
卷 13, 期 20, 页码 10501-10515出版社
ROYAL SOC CHEMISTRY
DOI: 10.1039/d2fo02125k
关键词
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资金
- Forestry Science and Technology Innovation and Promotion Project of Jiangsu Province [LYKJ [2021]40]
- Project of Science and Technology Support Plan of Taizhou [SNY20210022]
- Jiangsu Agriculture Science and Technology Innovation Fund (JASTIF) [CX (21)3174]
- Priority Academic Program Development of Jiangsu Higher Education Institutions
A novel heteropolysaccharide (EPS 7-4) was isolated from Lactobacillus crispatus in this study. EPS 7-4 exhibited excellent antibacterial and anti-inflammatory abilities, inhibiting the proliferation, biofilm formation, and virulence factor gene expression of Salmonella typhimurium. It also effectively restricted bacterial translocation, upregulated the abundance of beneficial bacteria, and alleviated severe inflammatory response in the intestinal tract of mice.
In this study, a novel heteropolysaccharide (EPS 7-4) with a molecular weight of 53 387 Da was isolated from Lactobacillus crispatus, and it was mainly composed of mannose (36.9%) and glucose (30.8%). EPS 7-4 showed excellent inhibitory effects on the proliferation, biofilm formation, and virulence factor gene expression of Salmonella typhimurium (S. typhimurium) by disrupting the integrity of the bacterial wall. Furthermore, EPS 7-4 can effectively restrict bacterial translocation, upregulate the abundance of Lactobacillus spp. and Bifidobacterium spp., and alleviate the S. typhimurium induced severe inflammatory response in the intestinal tract of mice. Besides, we demonstrated that EPS 7-4 can protect mice by inhibiting S. typhimurium induced pyroptosis, with the mechanism that EPS 7-4 affects ASC oligomerization during inflammasome-mediated pyroptosis. Therefore, due to its excellent anti-bacterial and anti-inflammatory abilities, EPS 7-4 is a promising health regulator owing to its excellent antibacterial and anti-inflammatory abilities.
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