Semi-3D cultures using Laminin 221 as a coating material for human induced pluripotent stem cells

It was previously believed that human induced pluripotent stem cells (hiPSCs) did not show adhesion to the coating material Laminin 221, which is known to have specific affinity for cardiomyocytes. In this study, we report that human mononuclear cell-derived hiPSCs, established with Sendai virus vector, form peninsular-like colonies rather than embryonic stem cell-like colonies; these peninsular-like colonies can be passaged more than 10 times after establishment. Additionally, initialization-deficient cells with residual Sendai virus vector adhered to the coating material Laminin 511 but not to Laminin 221. Therefore, the expression of undifferentiated markers tended to be higher in hiPSCs established on Laminin 221 than on Laminin 511. On Laminin 221, hiPSCs15M66 showed a semi-floating colony morphology. The expression of various markers of cell polarity was significantly lower in hiPSCs cultured on Laminin 221 than in hiPSCs cultured on Laminin 511. Furthermore, 201B7 and 15M66 hiPSCs showed 3D cardiomyocyte differentiation on Laminin 221. Thus, the coating material Laminin 221 provides semi-floating culture conditions for the establishment, culture and induced differentiation of hiPSCs.

Automated summary

Previous belief regarding the compatibility of human induced pluripotent stem cells (hiPSCs) and the coating material Laminin 221 has been challenged by this study. It was found that hiPSCs derived from human mononuclear cells can form peninsular-like colonies when cultured on Laminin 221, and these colonies can be passaged multiple times. The expression of undifferentiated markers tends to be higher in hiPSCs cultured on Laminin 221, and these cells also showed the ability to differentiate into cardiomyocytes on Laminin 221.