4.6 Article

Highly sensitive benzothiazole-based chemosensors for detection and bioimaging of peroxynitrite in living cells

期刊

RSC ADVANCES
卷 12, 期 43, 页码 27933-27939

出版社

ROYAL SOC CHEMISTRY
DOI: 10.1039/d2ra04549d

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资金

  1. Natural Science Foundation of Chaohu University [XLY-202002, XLY-202212, XLY-202211]
  2. Discipline Construction Quality Improvement Project [kj21fdzy03]
  3. Chaohu University [KYQD-202006]
  4. Key Research and Development Program of Anhui Province [2022a05020019]
  5. National Nature Science Foundation of China [82003208]
  6. Science and Technology Project of Jilin Province Education Department [JJKH20221069KJ]
  7. Natural Science Foundation of Anhui Province [2108085QC147]

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It is of great significance to detect and image peroxynitrite (ONOO-) in vitro and in vivo with high selectivity and sensitivity. In this study, two simple benzothiazole-based fluorescent chemosensors, BS1 and BS2, were reported. Both probes could quickly sense ONOO- under physiological pH and showed a remarkable turn-on fluorescence signal. BS1, with a diphenyl phosphonate unit, exhibited higher selectivity for ONOO- than BS2. Additionally, BS1 was successfully employed to detect and image ONOO- in HepG2 cells with lower cytotoxicity and pH-stability. Furthermore, BS1 was used to showcase drug-induced hepatotoxicity and evaluate the remediation effect of GSH, demonstrating its great potential for detecting ONOO-.
It is well accepted that peroxynitrite (ONOO-) plays a crucial role in various physiological and pathological processes. Thus, the detection and imaging of ONOO- in vitro and in vivo with high selectivity and sensitivity is of great significance. Here we report two simple benzothiazole-based fluorescent chemosensors, BS1 and BS2. Under physiological pH, both probes could quickly sense ONOO- with a remarkable turn-on fluorescence signal at 430 nm. The limit of detection (LOD) of BS1 and BS2 toward ONOO- was 12.8 nM and 25.2 nM, respectively, much lower than the reported values. Experimental results indicated that BS1 with a diphenyl phosphonate unit presented higher selectivity for ONOO- than BS2. Furthermore, based on the advantages of lower cytotoxicity and pH-stabilities of BS1, probe BS1 was successfully employed to detect and image ONOO- in HepG2 cells. More importantly, we used BS1 to successfully showcase drug-induced hepatotoxicity via imaging ONOO- upregulated by acetaminophen (APAP), and also evaluated the remediation effect of GSH. All the results illustrated that the fluorescent probe BS1 has great potential for the detection of ONOO- and to further uncover the roles of ONOO- during the drug-induced liver injury (DILI) process.

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