期刊
PHYSICAL REVIEW APPLIED
卷 6, 期 5, 页码 -出版社
AMER PHYSICAL SOC
DOI: 10.1103/PhysRevApplied.6.054006
关键词
-
资金
- National Institutes of Health [NHGRI R21HG005100-01]
- Oxford Nanopore Technologies, Ltd.
This paper investigates how faithfully an electrospray mass spectrometer reports the order of monomers of a single biopolymer in the context of two sequencing strategies. We develop a simplified one-dimensional theoretical model of the dynamics of Brownian particles in the Taylor cone of an electropray source, where free monomers drift towards the apex in an elongational force gradient. The likelihood that neighboring particles will invert their order decreases near the apex because the strength of the force gradient increases. Neighboring monomers on a stretched biopolymer should be cleaved by photo-fragmentation within about 3 nm of the apex if they are to enter the mass spectrometer in sequence with 95% probability under typical experimental conditions. Alternatively, if the monomers are cleaved processively at milliseconds-long intervals by an enzyme, their sequence will be faithfully reported with 95% confidence if the enzyme is within about 117 nm of the apex.
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