期刊
PEERJ
卷 4, 期 -, 页码 -出版社
PEERJ INC
DOI: 10.7717/peerj.2017
关键词
Phalaenopsis; Draft genome; PhAGL6b; Flower organ development; Flowering time
资金
- Agriculture and Food Agency, Council of Agriculture, Taiwan [102AS-9.1.1-FD-Z2(1), 103AS-9.1.1-FD-Z2(1), 104AS-9.1.1-FD-Z2(1)]
The Phalaenopsis orchid is an important potted flower of high economic value around the world. We report the 3.1 Gb draft genome assembly of an important winter flowering Phalaenopsis 'KHM190' cultivar. We generated 89.5 Gb RNA-seq and 113 million sRNA-seq reads to use these data to identify 41,153 protein-coding genes and 188 miRNA families. We also generated a draft genome for Phalaenopsis pulcherrima 'B8802,' a summer flowering species, via resequencing. Comparison of genome data between the two Phalaenopsis cultivars allowed the identification of 691,532 single-nucleotide polymorphisms. In this study, we reveal that the key role of PhAGL6b in the regulation of labellum organ development involves alternative splicing in the big lip mutant. Petal or sepal overexpressing PhAGL6b leads to the conversion into a lip-like structure. We also discovered that the gibberellin pathway that regulates the expression of flowering time genes during the reproductive phase change is induced by cool temperature. Our work thus depicted a valuable resource for the flowering control, flower architecture development, and breeding of the Phalaenopsis orchids.
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