☆ 4.6 Article

Short-range ENDOR distance measurements between Gd(iii) and trifluoromethyl labels in proteins

PHYSICAL CHEMISTRY CHEMICAL PHYSICS (2022)

相关参考文献

注意：仅列出部分参考文献，下载原文获取全部文献信息。

Article Chemistry, Physical

Application of W-band 19F electron nuclear double resonance (ENDOR) spectroscopy to distance measurement using a trityl spin probe and a fluorine label

N. B. Asanbaeva et al.

Summary: In this study, the suitability of high-field ENDOR spectroscopy in the W-band for triarylmethyl and fluorine label pairs was investigated using newly synthesized model compounds. The results showed that strong magnetic fields allowed for distinguishing nuclear frequencies of F-19 and protons with sufficient resolution. The study also applied this approach to investigate the thiol exchange process between triarylmethyl-labeled and F-19-labeled human serum albumin molecules.

PHYSICAL CHEMISTRY CHEMICAL PHYSICS (2022)

添加到收藏夹

Article Chemistry, Multidisciplinary

19F Electron-Nuclear Double Resonance Reveals Interaction between Redox-Active Tyrosines across the α/β Interface of E. coli Ribonucleotide Reductase

Andreas Meyer et al.

Summary: Ribonucleotide reductases (RNRs) play a key role in DNA replication and repair by catalyzing the reduction of ribonucleotides. In Escherichia coli, RNRs undergo a multistep radical transfer process involving proton-coupled electron transfer. Advanced EPR spectroscopy has been used to study this mechanism and revealed important insights into the behavior of the RNR enzyme complex.

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY (2022)

添加到收藏夹

Article Biochemical Research Methods

Resolution of chemical shift anisotropy in 19F ENDOR spectroscopy at 263 GHz/9.4 T

Annemarie Kehl et al.

Summary: Pulsed F-19 ENDOR spectroscopy is a selective method for measuring distances in structural biology, with pronounced asymmetric features observed at 9.4 T due to chemical shift anisotropy. CSA parameters are consistent with DFT predicted values and can be extracted from experimental data simulations.

JOURNAL OF MAGNETIC RESONANCE (2021)

添加到收藏夹

Article Chemistry, Multidisciplinary

Through-Space Scalar 19F-19F Couplings between Fluorinated Noncanonical Amino Acids for the Detection of Specific Contacts in Proteins

Henry W. Orton et al.

Summary: In this study, it was demonstrated that fluorine atoms in noncanonical amino acids positioned in the hydrophobic core or on the surface of a protein can be linked by scalar through-space F-19-F-19 couplings, even if the F-19 spins are separated by more than the van der Waals distance. The use of fluorinated amino acids and F-19-F-19 interactions offer a straightforward way to probe the spatial proximity of selected sites in proteins, without requiring H-1 NMR resonance assignments.

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY (2021)

添加到收藏夹

Article Chemistry, Multidisciplinary

A Chiral Lanthanide Tag for Stable and Rigid Attachment to Single Cysteine Residues in Proteins for NMR, EPR and Time-Resolved Luminescence Studies

Iresha D. Herath et al.

Summary: The study introduces a new stable chiral Ln(III) tag, known as C12, for site-specific attachment to proteins, enabling protein probing through various spectroscopic techniques. The C12 tag exhibits enhanced reactivity towards selenocysteine, offering a pathway for selectively tagging selenocysteine in proteins containing cysteine residues. With Tb-III or Tm-III ions, the C12 tag can generate pseudocontact shifts in protein NMR spectra, providing a rigid tether between lanthanide and protein for interpretation of the shifts and distance measurements in double electron-electron resonance experiments. Additionally, the Tb-III complex upon reaction with cysteine or thiol compounds shows a significant enhancement in luminescence quantum yield, making it a sensitive luminescence probe for time-resolved FRET assays and enzyme reaction monitoring.

CHEMISTRY-A EUROPEAN JOURNAL (2021)

添加到收藏夹

Article Chemistry, Physical

Determining electron-nucleus distances and Fermi contact couplings from ENDOR spectra†

Stephan Pribitzer et al.

Summary: The study introduces a novel regularization approach for analyzing and interpreting ENDOR spectra to identify ligand protons in metal compounds. The use of smoothness regularization, common in DEER spectroscopy, is found not suitable for ENDOR analysis.

PHYSICAL CHEMISTRY CHEMICAL PHYSICS (2021)

添加到收藏夹

Article Chemistry, Multidisciplinary

Measurement of Angstrom to Nanometer Molecular Distances with 19F Nuclear Spins by EPR/ENDOR Spectroscopy

Andreas Meyer et al.

ANGEWANDTE CHEMIE-INTERNATIONAL EDITION (2020)

添加到收藏夹

Article Biochemical Research Methods

UWB DEER and RIDME distance measurements in Cu(II)-Cu(II) spin pairs

Frauke D. Breitgoff et al.

JOURNAL OF MAGNETIC RESONANCE (2019)

添加到收藏夹

Article Chemistry, Physical

On the Use of Q-Band Double Electron-Electron Resonance To Resolve the Relative Orientations of Two Double Histidine-Bound Cu2+ Ions in a Protein

Austin Gamble Jarvi et al.

JOURNAL OF PHYSICAL CHEMISTRY B (2018)

添加到收藏夹

Article Chemistry, Physical

Gd3+-Gd3+ distances exceeding 3 nm determined by very high frequency continuous wave electron paramagnetic resonance

Jessica A. Clayton et al.

PHYSICAL CHEMISTRY CHEMICAL PHYSICS (2017)

添加到收藏夹

Article Chemistry, Physical

1H and 19F spin-lattice relaxation and CH3 or CF3 reorientation in molecular solids containing both H and F atoms

Peter A. Beckmann et al.

JOURNAL OF CHEMICAL PHYSICS (2016)

添加到收藏夹

Article Chemistry, Physical

EPR characterization of Mn(II) complexes for distance determination with pulsed dipolar spectroscopy

Katharina Keller et al.

PHYSICAL CHEMISTRY CHEMICAL PHYSICS (2016)

添加到收藏夹

Article Chemistry, Physical

Exploiting orientation-selective DEER: determining molecular structure in systems containing Cu(II) centres

Alice M. Bowen et al.

PHYSICAL CHEMISTRY CHEMICAL PHYSICS (2016)

添加到收藏夹

Article Chemistry, Inorganic & Nuclear

Mn(II) tags for DEER distance measurements in proteins via C-S attachment

Andrea Martorana et al.

DALTON TRANSACTIONS (2015)

添加到收藏夹

Article Biochemical Research Methods

High-field ELDOR-detected NMR study of a nitroxide radical in disordered solids: Towards characterization of heterogeneity of microenvironments in spin-labeled systems

Anna Nalepa et al.

JOURNAL OF MAGNETIC RESONANCE (2014)

添加到收藏夹

Article Chemistry, Physical

RIDME Spectroscopy with Gd(III) Centers

Sahand Razzaghi et al.

JOURNAL OF PHYSICAL CHEMISTRY LETTERS (2014)

添加到收藏夹

Review Chemistry, Physical

DEER Distance Measurements on Proteins

Gunnar Jeschke

ANNUAL REVIEW OF PHYSICAL CHEMISTRY, VOL 63 (2012)

添加到收藏夹

Review Chemistry, Physical

Current applications of 19F NMR to studies of protein structure and dynamics

Julianne L. Kitevski-LeBlanc et al.

PROGRESS IN NUCLEAR MAGNETIC RESONANCE SPECTROSCOPY (2012)

添加到收藏夹

Article Biochemical Research Methods

DOTA-Amide Lanthanide Tag for Reliable Generation of Pseudocontact Shifts in Protein NMR Spectra

Bim Graham et al.

BIOCONJUGATE CHEMISTRY (2011)

添加到收藏夹

Article Chemistry, Multidisciplinary

Gadolinium Tagging for High-Precision Measurements of 6 nm Distances in Protein Assemblies by EPR

Hiromasa Yagi et al.

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY (2011)

添加到收藏夹

Article Biochemical Research Methods

Distance measurements in model bis-Gd(III) complexes with flexible bridge. Emulation of biological molecules having flexible structure with Gd(III) labels attached

A. Potapov et al.

JOURNAL OF MAGNETIC RESONANCE (2010)

添加到收藏夹

Article Chemistry, Multidisciplinary

Nanometer-Scale Distance Measurements in Proteins Using Gd3+ Spin Labeling

Alexey Potapov et al.

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY (2010)

添加到收藏夹

Article Chemistry, Multidisciplinary

Flexibility of Shape-Persistent Molecular Building Blocks Composed of p-Phenylene and Ethynylene Units

Gunnar Jeschke et al.

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY (2010)

添加到收藏夹

Article Biochemical Research Methods

A pulsed EPR method to determine distances between paramagnetic centers with strong spectral anisotropy and radicals: The dead-time free RIDME sequence

Sergey Milikisyants et al.

JOURNAL OF MAGNETIC RESONANCE (2009)

添加到收藏夹

Article Biochemical Research Methods

Nuclear relaxation effects in davies ENDOR variants

John J. L. Morton et al.

JOURNAL OF MAGNETIC RESONANCE (2008)

添加到收藏夹

Article Biochemical Research Methods

Biomolecular solid state NMR with magic-angle spinning at 25 K

Kent R. Thurber et al.

JOURNAL OF MAGNETIC RESONANCE (2008)

添加到收藏夹

Article Chemistry, Multidisciplinary

Fluorine in a native protein environment - How the spatial demand and polarity of fluoroalkyl groups affect protein folding

Christian Jaeckel et al.

ANGEWANDTE CHEMIE-INTERNATIONAL EDITION (2006)

添加到收藏夹

Article Biochemical Research Methods

EasySpin, a comprehensive software package for spectral simulation and analysis in EPR

S Stoll et al.

JOURNAL OF MAGNETIC RESONANCE (2006)

添加到收藏夹

Article Chemistry, Physical

Distance measurements between paramagnetic centers and a planar object by matrix Mims electron nuclear double resonance -: art. no. 024515

PP Zänker et al.

JOURNAL OF CHEMICAL PHYSICS (2005)

添加到收藏夹

Article Biochemistry & Molecular Biology

Fluorine interactions at the thrombin active site:: Protein backbone fragments H-Cα-C=O comprise a favorable C-F environment and interactions of C-F with electrophiles

JA Olsen et al.

CHEMBIOCHEM (2004)

添加到收藏夹

Review Multidisciplinary Sciences

Electron spin resonance in studies of membranes and proteins

PP Borbat et al.

SCIENCE (2001)

添加到收藏夹

Article Biochemical Research Methods

Dead-time free measurement of dipole-dipole interactions between electron spins

M Pannier et al.

JOURNAL OF MAGNETIC RESONANCE (2000)

添加到收藏夹

© Peeref 2019-2024. All rights reserved.